Molecular cloning and characterization of 1-Cys and 2-Cys peroxiredoxins from the bumblebee Bombus ignitus

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Zhigang HuByung Rae Jin

Abstract

We cloned and characterized two peroxiredoxins (Prxs), BiPrx1 (a 1-Cys Prx) and BiTPx1 (a 2-Cys Prx) from the bumblebee Bombus ignitus. The BiPrx1 gene consists of 5 exons, encoding 220 amino acid residues with one conserved cysteine residue. The BiTPx1 gene consists of three exons, encoding 195 amino acid residues with 2 conserved cysteine residues. Recombinant BiPrx1 (27 kDa) and BiTPx1 (25 kDa), expressed in baculovirus-infected insect Sf9 cells, reduced H2O2 in the presence of electrons donated by dithiothreitol. Unlike BiTPx1, however, BiPrx1 did not show reduction activity when thioredoxin was used as the electron donor. Both BiPrx1 and BiTPx1 protected super-coiled DNA from damage by metal-catalyzed oxidation (MCO) in vitro. Tissue distribution analyses showed the presence of BiPrx1 and BiTPx1 in the fat body, midgut, muscle and epidermis, but not in the hemolymph, suggesting that BiPrx1 and BiTPx1 are not secretable. When H2O2 was injected into B. ignitus bees, BiPrx1 and BiTPx1 transcripts were acutely up-regulated in the fat body tissues. We also demonstrated the regulation of BiPrx1 and BiTPx1 expression via reduction of transcript levels in the fat body with RNA interference (RNAi). Under H2O2 overload, the RNAi-ind...Continue Reading

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Mar 15, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Iksoo KimByun Rae Jin
May 30, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yong Soo ChoiByung Rae Jin
May 1, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Young Joo KimByung Rae Jin
Oct 16, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Qiang WangRunhong Mu

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Citations

Apr 22, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yan YanBaohua Xu
Jul 8, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hu WanJianhong Li
Oct 20, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Bo Yeon KimByung Rae Jin
Jun 17, 2011·Archives of Insect Biochemistry and Physiology·Eun-Young YunTae-Won Goo
May 11, 2016·Archives of Insect Biochemistry and Physiology·André Fernando Ditondo MicasMárcia Maria Gentile Bitondi
Dec 28, 2018·PeerJ·Li Tian, Heather M Hines
Dec 2, 2020·International Journal of Biological Macromolecules·Patricia P AdrianiFelipe S Chambergo
Oct 29, 2020·Proceedings. Biological Sciences·Jason A RothmanPeter Graystock
Apr 6, 2018·Developmental and Comparative Immunology·Hee Geun ParkByung Rae Jin

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