Molecular cloning and characterization of a new gene, Oocyte-G1

Journal of Cellular Physiology
Yong Zhang, Ji Wu

Abstract

Oocytes are recognized as a source of regulatory molecules that influence follicular development through an array of actions on granulosa cells. Recently, more and more hormones and signaling molecules were identified during follicular developmental processes; however, the details about their functions are still unclear. During efforts to clone follicular development-related genes, we isolated a cDNA fragment by DDRT-PCR. To obtain cDNA 5'- and 3'-end sequences, we screened a mouse ovarian cDNA library. After screening the library, an open reading frame of 2,994 bp for the new gene (Oocyte-G1), which encodes a 997-residue protein, was cloned. Northern blot analysis revealed the presence of approximately 3.6 kb Oocyte-G1 mRNA in ovary, lung, kidney, testis and brain. Northern analysis of RNA from ovaries in vivo showed that Oocyte-G1 was weakly expressed on day 5 and at a moderate level on day 10. Thereafter, on day 15 or in adults (day 40), there was an increase in expression, followed by a decline in ovaries on day 20 or older (day 120). Furthermore, we studied the Ooctye-G1 protein by using the antiserum against a peptide sequence unique to this gene in Western blotting and immunolocalization. The antiserum recognized a promi...Continue Reading

References

Jan 1, 1991·International Review of Cytology·A N Hirshfield
Jan 1, 1994·Developmental Biology·A I PackerR F Bachvarova
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·J W Zimmermann, R M Schultz
Jan 1, 1995·Molecular Endocrinology·S A McGrathS J Lee
Nov 14, 1997·The Journal of Clinical Endocrinology and Metabolism·K OktayR G Gosden
Jan 1, 1997·British Medical Bulletin·R Gosden, N Spears
Aug 29, 1998·Molecular and Cellular Endocrinology·E A McGeeA J Hsueh
Dec 16, 1998·Molecular Endocrinology·J L DubeM M Matzuk
Jan 28, 1999·Molecular and Cellular Endocrinology·S Y Hsu, A J Hsueh
May 16, 2000·Biological Signals and Receptors·E A McGee
Aug 30, 2000·Molecular and Cellular Endocrinology·J E FortuneS Kito
Sep 19, 2000·Nature·D J SharpJ M Scholey
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·John J EppigFrank L Pendola
Feb 6, 2004·Current Biology : CB·J Nicholas BetleyJames O Deshler
Jul 15, 2004·Journal of Cell Science·Matthew S SavoianDavid M Glover
Oct 7, 2004·Current Opinion in Neurobiology·Nobutaka Hirokawa, Reiko Takemura
Feb 16, 2005·Nature Reviews. Neuroscience·Nobutaka Hirokawa, Reiko Takemura
Aug 9, 2005·Trends in Cell Biology·Harukata MikiNobutaka Hirokawa
Nov 24, 2005·Current Biology : CB·Gohta GoshimaRonald D Vale
May 24, 2006·Microscopy Research and Technique·Sandra CecconiMaria Grazia Palmerini

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Citations

May 14, 2010·Reproductive Biology and Endocrinology : RB&E·Zhao-juan YangJi Wu
Aug 16, 2013·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Maurizio BellaviaAttilio Ignazio Lo Monte
Dec 30, 2014·Endocrine Reviews·Katja HummitzschRaymond J Rodgers

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