Molecular cloning and characterization of a novel human J-domain protein gene (HDJ3) from the fetal brain

Journal of Human Genetics
Juxiang ChenYi Xie

Abstract

The J-domain is believed to be part of a chaperone involved in protein folding. From a fetal brain cDNA library, we isolated a cDNA of 3249 bp encoding a novel human J-domain protein, which was named as HDJ3. The expression pattern of HDJ3 was examined by reverse transcription/polymerase chain reaction, which suggested that the transcripts were highly expressed in human pancreas and selectively expressed in human brain, lung, liver, skeletal muscle and kidney. The results also showed that a probable splice variant of HDJ3 gene might exist. The HDJ3 gene was located on human chromosome 12q13.1-12q13.2 and consisted of seven exons spanning 8593 bp of the human genome. PSORT analysis indicated that the HDJ3 gene contained a transmembrane domain. The putative protein of the HDJ3 gene was highly homologous to rat dopamine-receptor-interacting protein, suggesting that it was a novel member of the molecular chaperone family and functionally related to dopamine signal transduction.

References

Jan 1, 1991·Annual Review of Biochemistry·R J Ellis, S M van der Vies
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y Y Huang, E R Kandel
May 19, 1998·Cell Stress & Chaperones·M E Cheetham, A J Caplan
Jun 30, 1998·Trends in Biochemical Sciences·W L Kelley
Oct 16, 2002·The Journal of Biological Chemistry·Yuchen MaEvan Eisenberg

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Citations

Jan 21, 2014·International Journal of Molecular Sciences·Benoît T Roux, Graeme S Cottrell
Jan 8, 2016·Journal of Virology·Leonia BozzaccoMargaret R MacDonald
Nov 6, 2004·The Journal of Biological Chemistry·Jessica RobertMaria Angeles Ventura
Mar 17, 2007·The Journal of Biological Chemistry·Denis J DupréTerence E Hébert

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