Molecular cloning and characterization of a novel ABC transporter gene in the human pathogen Trichophyton rubrum

Medical Mycology
Eliane P CervelattiNilce M Martinez-Rossi

Abstract

A gene encoding an ABC transporter in the dermatophyte Trichophyton rubrum, TruMDR1, was cloned by PCR using degenerate primers. The open reading frame of TruMDR1 is 4838 bp long and the deduced amino acid sequence shows high homology with ABC transporters involved in drug efflux of other fungi. The effect of chemicals on the expression level of mRNAs of this gene was analysed by Northern blot. An increase in expression level was observed when the fungus was exposed to ethidium bromide, ketoconazole, cycloheximide, fluconazole, griseofulvin, imazalil and itraconazole, suggesting the participation of this gene in drug efflux in this dermatophyte. The identification of a gene potentially involved in cellular detoxification in a pathogenic fungus is the first step towards knowing molecular events related to antifungal resistance.

References

Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Apr 1, 1995·Clinical Microbiology Reviews·I Weitzman, R C Summerbell
Aug 1, 1995·Journal of the American Academy of Dermatology·M E GrossmanD N Silvers
Mar 1, 1995·Protein Science : a Publication of the Protein Society·B RostC Sander
Apr 28, 1997·Molecular & General Genetics : MGG·G Del SorboM A De Waard
Aug 26, 1998·Methods in Enzymology·D Taglicht, S Michaelis
Jun 19, 2002·Molecular Microbiology·Alex A Neyfakh
Aug 23, 2002·Functional & Integrative Genomics·Benoît De HertoghAndré Goffeau
Mar 26, 2003·The British Journal of Dermatology·D T RobertsUNKNOWN British Association of Dermatologists
Aug 9, 2003·Molecular Plant-microbe Interactions : MPMI·Ioannis StergiopoulosMaarten A De Waard
Sep 27, 2003·The British Journal of Dermatology·R K Scher, R Baran
Nov 8, 2003·Journal of Clinical Microbiology·Ran Nir-PazItzhack Polacheck

❮ Previous
Next ❯

Citations

Jan 1, 2009·Clinical, Cosmetic and Investigational Dermatology·Jason G Newland, Susan M Abdel-Rahman
May 15, 2008·Mycopathologia·Nilce M Martinez-RossiAntonio Rossi
Feb 20, 2016·Journal of the American Podiatric Medical Association·Mahmoud Ghannoum
Mar 23, 2013·Journal de mycologie médicale·E R RodriguesR C L R Pietro
Dec 21, 2010·Revista iberoamericana de micología·Gioconda San-Blas, Eva Burger
Mar 3, 2007·International Journal of Antimicrobial Agents·Daniel de Assis SantosPatrícia Silva Cisalpino
Jan 15, 2009·Journal of Medical Microbiology·Fernanda C A MaranhãoNilce M Martinez-Rossi
Apr 12, 2007·FEMS Microbiology Letters·Fernanda G PaiãoNilce M Martinez-Rossi
May 18, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Huazhi HuangMeiying Hu
Jul 17, 2014·Drug Discovery Today. Technologies·Michael ViereckGerhard F Ecker
Apr 29, 2016·Journal of Medical Microbiology·Maíra Pompeu MartinsNilce Maria Martinez-Rossi
Mar 19, 2013·Biochemical Pharmacology·Jorge L M RuizSérgio P Bydlowski
Feb 9, 2017·Frontiers in Microbiology·Liliana ScorzoniAna M Fusco-Almeida
Jan 13, 2015·BMC Genomics·Gabriela Felix PersinotiNilce Maria Martinez-Rossi
Feb 13, 2020·Journal of Applied Microbiology·S GnatA Nowakiewicz
Sep 11, 2018·Skin Appendage Disorders·Aditya K GuptaNeil H Shear
Sep 11, 2019·Antimicrobial Agents and Chemotherapy·Michel MonodTsuyoshi Yamada
Jun 14, 2018·Frontiers in Microbiology·Nilce M Martinez-RossiAntonio Rossi
Dec 15, 2010·Anais Brasileiros De Dermatologia·Nalu Teixera de Aguiar PeresNilce Maria Martinez-Rossi
Mar 1, 2019·Journal of Fungi·Michel Monod, Bruno Méhul
Feb 3, 2021·Microbiological Research·Ján Víglaš, Petra Olejníková
Jul 6, 2021·Indian Journal of Dermatology, Venereology and Leprology·Shyam B VermaResham Vasani

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.