PMID: 9524069Apr 2, 1998Paper

Molecular cloning and characterization of a novel tissue-specific calpain predominantly expressed in the digestive tract

Biological Chemistry
H J LeeK Suzuki

Abstract

In the course of the genomic cloning of nCL-2, a stomach-specific calpain, we identified a genomic clone encoding a novel member of the calpain large subunit family and designated it 'nCL-4'. First, using exon sequences, we cloned the cDNA for mouse nCL-4. Based on this sequence, we also cloned the cDNAs for rat and human nCL-4. In the case of human nCL-4, the longest open reading frame encodes 690 amino acid residues (Mr 79095) with equal sequence similarities (50-55%) to both ubiquitous and organ-specific calpain large subunits from mammals. The deduced amino acid sequence revealed that nCL-4 is highly conserved among mammals. nCL-4 can be aligned without significant deletions or insertions, and, thus, like other calpains, can be divided into four domains (I-IV). The significant similarity of domains II and IV to those in conventional calpain large subunits suggests the potential protease activity and Ca2+-binding ability of nCL-4. Northern blot analysis revealed that the mRNA for nCL-4 is expressed predominantly in stomach and small intestine but not in uterus, suggesting specialized functions of nCL-4 in the digestive tract. When overexpressed in COS-7 cells, a specific band for nCL-4 was detected. In addition, the gene cod...Continue Reading

References

May 1, 1992·Nature Genetics·R WaterstonN Halloran
Nov 10, 1992·Biochimica Et Biophysica Acta·H Sorimachi, K Suzuki
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S J DelaneyG L Miklos
Jul 1, 1991·Physiological Reviews·D E Croall, G N DeMartino
Apr 18, 1994·FEBS Letters·H SorimachiK Suzuki

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Citations

Jun 7, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C LadratJ Fleurence
Jan 31, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yoshiyuki TamadaThomas R Shearer
Aug 23, 2001·Trends in Molecular Medicine·Y Huang, K K Wang
Mar 10, 2009·The Journal of Biological Chemistry·Alicia De MariaSteven Bassnett
Jan 30, 2004·Molecular and Cellular Biology·Tanna FranzT Neil Dear
Oct 9, 2008·Journal of Cell Science·Vishnu V UndyalaKaren A Beningo
Jun 15, 2011·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Hiroyuki SorimachiYasuko Ono
Sep 26, 2006·Drug Discovery Today·Maria E SaezAgustin Ruiz
Aug 2, 2003·Biochemical and Biophysical Research Communications·Mitsuyoshi AzumaThomas R Shearer
Aug 6, 1999·Experimental Eye Research·Y NakamuraT R Shearer
Nov 30, 2002·Experimental Eye Research·Kanako Sakamoto-MizutaniThomas R Shearer
Jun 19, 2008·Biochimica Et Biophysica Acta·Taku OzakiSei-Ichi Ishiguro
Feb 11, 2015·European Journal of Cell Biology·Joanna ObaczRoman Hrstka
Jun 3, 2000·Japanese Journal of Cancer Research : Gann·Y YoshikawaI Kato
Sep 7, 2017·GigaScience·Jinjie DuanTobias Wang
Jul 5, 2003·Physiological Reviews·Darrell E GollJinyang Cong
Mar 23, 2017·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Jatinder SinghCandace M Kammerer
Feb 6, 2021·Biochimica Et Biophysica Acta. Proteins and Proteomics·Simone SpinozziIsabelle Richard

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