Molecular cloning and characterization of a novel human classic cadherin homologous with mouse muscle cadherin.

The Journal of Biological Chemistry
Y ShimoyamaS Hirohashi

Abstract

We used a novel cDNA cloning method based on the cadherin-beta-catenin protein interaction and identified a new human classic-type cadherin, which we named cadherin-15, from adult brain and skeletal muscle cDNA libraries. Sequence analysis revealed that this cadherin was closely related to mouse muscle cadherin and seemed to be its human counterpart. However, its deduced amino acid sequence differed from that of mouse muscle cadherin in that it had an extra 31-amino acid sequence at its C terminus that has been found neither in mouse muscle cadherin nor in any other known classic cadherin. Analysis of cadherin-15 protein expressed in L fibroblasts showed that it was cleaved proteolytically, expressed on the cell surfaces as a mature form of about 124-kDa, and functioned as a cell-cell adhesion molecule in a homophilic and specific manner, but Ca2+ did not protect it against degradation by trypsin. Our findings also suggest that cadherin-15 mediates cell-cell adhesion with a binding strength comparable to that of E-cadherin.

References

Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M DonaliesA Starzinski-Powitz
May 1, 1991·The Journal of Cell Biology·E W NapolitanoL F Reichardt
Oct 1, 1990·The Journal of Cell Biology·M Ozawa, R Kemler
Oct 11, 1990·Nucleic Acids Research·R A Reid, J J Hemperly
May 1, 1990·Developmental Biology·O W BlaschukY Pouliot
May 1, 1990·The Journal of Cell Biology·R L HeimarkS M Schwartz
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M OzawaR Kemler
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Dec 1, 1987·Cell Structure and Function·K KatohH Kondoh
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K HattaM Takeichi
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R BertolottiG M Edelman
Jan 1, 1981·Annual Review of Biochemistry·S C Hubbard, R J Ivatt
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·O RoseW W Franke
Jul 21, 1995·The Journal of Biological Chemistry·R B ThomsonP S Aronson
Nov 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·O RoseA Starzinski-Powitz
Aug 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Y PouliotP C Holland
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·T OdaS Hirohashi
Dec 10, 1993·Science·B RubinfeldP Polakis
Feb 1, 1993·Molecular Biology Reports·M J BussemakersJ A Schalken
Oct 1, 1995·Current Opinion in Cell Biology·M Takeichi

❮ Previous
Next ❯

Citations

Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·Sabine BlaschkeGerd Klein
Feb 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J KawaguchiA Kudo
Jul 5, 2006·Archives of Histology and Cytology·Benio TsuchiyaKiyoshi Mukai
Feb 1, 2002·The Journal of Biological Chemistry·Stéphanie BibertDanielle Gulino-Debrac

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.