Molecular cloning and characterization of a platelet glycoprotein Ib-binding protein from the venom of Trimeresurus stejnegeri

Toxicon : Official Journal of the International Society on Toxinology
Wen-Hui Lee, Yun Zhang

Abstract

A platelet glycoprotein Ib-binding protein, termed TSV-GPIb-BP, was isolated from the venom of Trimeresurus stejnegeri. On SDS-polyacrylamide gel electrophoresis, TSV-GPIb-BP showed a single band with an apparent molecular weight of 28,000 and two distinct bands with apparent molecular weights of 16,000 and 15,000 under non-reducing and reducing conditions, respectively. cDNA clones containing the coding sequences for both TSV-GPIb-BP subunits were isolated and sequenced. The deduced amino acid sequences of TSV-GPIb-BP subunits were confirmed by N-terminal protein sequencing and trypsin-digested peptide mass fingerprinting. Interestingly, the alpha subunit of TSV-GPIb-BP is identical to that of alboaggregin-B, and the sequence identity of their beta subunits is 94.3%. TSV-GPIb-BP inhibited ristocetin-induced human platelet agglutination in platelet-rich plasma under lower dosages (<5 microg/ml). On the other hand, it directly aggregated washed human platelets in the absence of additional Ca2+ or any other cofactors under higher dosages (>5 microg/ml). This platelet aggregation activity was dose-dependently inhibited by specific GPIbalpha antibodies, but not by those antibodies against platelet GPIa, GPIIa, GPIIb and GPIIIa.

References

Oct 1, 1979·Agents and Actions·G Bereziat
Oct 16, 2001·Toxicon : Official Journal of the International Society on Toxinology·H G ZhaY Zhang

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Citations

Apr 25, 2006·Blood Cells, Molecules & Diseases·Xiao-Yan DuYun Zhang
Feb 21, 2006·Toxicon : Official Journal of the International Society on Toxinology·Shao-Gui WanYun Zhang
Feb 21, 2006·Toxicon : Official Journal of the International Society on Toxinology·Shao-Gui WanYun Zhang
May 9, 2012·Toxicon : Official Journal of the International Society on Toxinology·Lin ZengYun Zhang
Jan 25, 2011·Toxicon : Official Journal of the International Society on Toxinology·ZhongMing ChenYun Zhang
Oct 27, 2004·Toxicon : Official Journal of the International Society on Toxinology·Hong TaiYun Zhang
May 7, 2005·Toxicon : Official Journal of the International Society on Toxinology·Qiumin LuKenneth J Clemetson
Jun 1, 2007·Platelets·Jaradpong ArpijuntarangkoonTanin Intragumtornchai
Dec 7, 2005·Toxicon : Official Journal of the International Society on Toxinology·Shu-Rong ZhongYun Zhang

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