PMID: 9450971Apr 4, 1998Paper

Molecular cloning and characterization of a radial spoke head protein of sea urchin sperm axonemes: involvement of the protein in the regulation of sperm motility

Molecular Biology of the Cell
D GingrasC Gagnon

Abstract

Monoclonal antibodies raised against axonemal proteins of sea urchin spermatozoa have been used to study regulatory mechanisms involved in flagellar motility. Here, we report that one of these antibodies, monoclonal antibody D-316, has an unusual perturbating effect on the motility of sea urchin sperm models; it does not affect the beat frequency, the amplitude of beating or the percentage of motile sperm models, but instead promotes a marked transformation of the flagellar beating pattern which changes from a two-dimensional to a three-dimensional type of movement. On immunoblots of axonemal proteins separated by SDS-PAGE, D-316 recognized a single polypeptide of 90 kDa. This protein was purified following its extraction by exposure of axonemes to a brief heat treatment at 40 degrees C. The protein copurified and coimmunoprecipitated with proteins of 43 and 34 kDa, suggesting that it exists as a complex in its native form. Using D-316 as a probe, a full-length cDNA clone encoding the 90-kDa protein was obtained from a sea urchin cDNA library. The sequence predicts a highly acidic (pI = 4.0) protein of 552 amino acids with a mass of 62,720 Da (p63). Comparison with protein sequences in databases indicated that the protein is re...Continue Reading

References

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D LuckB Huang
Sep 1, 1992·Molecular and Cellular Biology·A M CurryJ L Rosenbaum
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1987·The Journal of Cell Biology·W C EarnshawD W Cleveland
Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B LapeyreF Amalric
Mar 1, 1984·The Journal of Cell Biology·D J Luck
Jan 1, 1981·The Journal of Cell Biology·B HuangD J Luck
Oct 1, 1995·Trends in Genetics : TIG·S K Dutcher
Dec 1, 1994·The Journal of Cell Biology·D R HowardW S Sale
Jan 1, 1993·Cell Motility and the Cytoskeleton·A M Curry, J L Rosenbaum
Jan 1, 1996·Cell Motility and the Cytoskeleton·J CossonC Gagnon
Jan 13, 1997·The Journal of Cell Biology·G Habermacher, W S Sale

❮ Previous
Next ❯

Citations

May 19, 2011·Molecular Human Reproduction·Kazuo Inaba
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·V P SahJ H Brown
Mar 10, 2001·Biochemical and Biophysical Research Communications·M ErikssonN Carey
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·François ChauvignéJoan Cerdà
Jun 15, 2014·Journal of Proteomics·Omar Mendoza-PorrasMichelle L Colgrave
Feb 26, 2003·Cell Motility and the Cytoskeleton·Christian Cibert
Apr 19, 2015·Reproductive Biology and Endocrinology : RB&E·Ashok AgarwalEdmund Sabanegh
Oct 23, 2001·Cell Motility and the Cytoskeleton·C Cibert
Mar 14, 2018·Journal of Animal Science·Agnieszka MostekMariola A Dietrich
Aug 29, 2006·Journal of Biochemistry·Hironori UenoOsamu Numata
Feb 23, 2002·The Journal of Biological Chemistry·Celine CharvetMarcel Deckert
May 9, 2001·The Journal of Biological Chemistry·D Gineitis, R Treisman
Oct 5, 2001·The Journal of Biological Chemistry·I GrossJ D Licht
Jan 25, 2005·The Journal of Biological Chemistry·Daniel WhiteClaude Gagnon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved