Molecular cloning and characterization of CDEP, a novel human protein containing the ezrin-like domain of the band 4.1 superfamily and the Dbl homology domain of Rho guanine nucleotide exchange factors

Biochemical and Biophysical Research Communications
Y KoyanoY Kato

Abstract

A cDNA for a novel human protein named CDEP was cloned using the subtractive hybridization method between dedifferentiated cartilage cells and overtly differentiated cartilage cells. CDEP cDNA contained an open reading frame encoding 1,045 amino acids in a total length of 3.4 kb. The deduced amino acid sequence revealed that a single polypeptide contained the ezrin-like domain, which is found in cytoskeleton-associated proteins of the band 4.1 superfamily, and the Dbl homology (DH) and pleckstrin homology (PH) domains, which are conserved in the Rho GEF (guanine nucleotide exchange factor) family. Northern blot analysis demonstrated that CDEP mRNA was expressed not only in the differentiated chondrocytes but also in various fetal and adult tissues. Since members of the band 4.1 superfamily and the Rho GEF family are crucial for microfilament organization, the novel protein CDEP may be involved in the adhesion, proliferation, and differentiation of some cell types including chondrocytes via changes in the cytoskeleton.

Citations

Nov 3, 2011·European Journal of Human Genetics : EJHG·Marta CzugalaMarzena Gajecka
Dec 5, 2012·The Journal of Cell Biology·Lucas Cheadle, Thomas Biederer
Apr 14, 2009·American Journal of Medical Genetics. Part a·Maria KirchhoffMargarita Stefanova
Jan 17, 2004·The Journal of Biological Chemistry·Guoyan WangFrank Beier
Nov 16, 2005·Nature Neuroscience·Toshihiko ToyofukuHitoshi Kikutani
Apr 6, 2005·World Journal of Gastroenterology : WJG·Zuo-Biao YuanSheng-Dao Zhang
Aug 13, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Noriko TakegaharaAtsushi Kumanogoh
Jun 29, 2017·Molecular Medicine Reports·Xiuhua JiaXiangming Guo
Jul 12, 2018·Scientific Reports·Yi-Chun KuoXuewu Zhang
Jun 27, 2020·Molecular Genetics & Genomic Medicine·Francesca CucinottaAntonio M Persico
Jul 16, 2019·The EMBO Journal·Fotine LibanjeFanny Jaulin
Oct 3, 1998·Trends in Biochemical Sciences·A H ChishtiK B Hoover

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