Molecular cloning and characterization of cDNA encoding fibrinolytic enzyme-3 from earthworm Eisenia foetida

Acta Biochimica Et Biophysica Sinica
Guo-Qing DongYuwen Cong

Abstract

The earthworm fibrinolytic enzyme-3 (EFE-3, GenBank accession No: AY438622), from the earthworm Eisenia foetida, is a component of earthworm fibrinolytic enzymes. In this study, cDNA encoding the EFE-3 was cloned by RT-PCR. The cDNA contained an open reading frame of 741 nucleotides, which encoded a deduced protein of 247 amino acid residues, including signal sequences. EFE-3 showed a high degree of homology to earthworm (Lumbricus rebullus) proteases F-III-1, F-III-2, and bovine trypsin. The recombinant EFE-3 was expressed in E. coli as inclusion bodies, and the gene encoding the native form of EFE-3 was expressed in COS-7 cells in the medium. Both the refolding product of inclusion bodies and the secreted protease could dissolve the artificial fibrin plate.

Citations

Jan 15, 2014·Evidence-based Complementary and Alternative Medicine : ECAM·Kevin Yueju WangDehu Liu
May 19, 2006·Preparative Biochemistry & Biotechnology·Xiaoling YuanYuwen Cong
Aug 12, 2020·International Journal of Biological Macromolecules·Ali Muhammed Moula Ali, Sri Charan Bindu Bavisetty

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