Molecular cloning and characterization of crustin from mud crab Scylla paramamosain.

Molecular Biology Reports
Chanprapa ImjongjirakSiriporn Sittipraneed

Abstract

Antimicrobial peptides (AMPs) are important components of the host innate immune response against microbial invasion. In the present study, we report the identification and characterization of a crustin (CrusSp) from the hemocyte of mud crab, Scylla paramamosain using an expressed sequence tag (EST) and rapid amplification cDNA end (RACE) approaches. Analysis of the nucleotide sequence revealed seven different variances of the CrusSp cDNA in mud crab. The open reading frame encodes a protein of 111 amino acids with 21 residues signal sequence. The predicted molecular mass of the mature protein (90 amino acids) is 10.27 kDa with an estimated pI of 8.54. Analysis of the protein domain features indicated typical conserved cysteine residues containing a single whey acidic protein (WAP) domain at the C-terminus. A neighbour-joining tree showed that S. paramamosain crustin is closely related to other crustin homologues, and displays the highest similarity to crustin antimicrobial peptide in shore crab Carcinus maenas. Four exons and three introns were identified within the 999 bp genomic DNA sequence of CrusSp. Tissue distribution analysis showed that CrusSp was highly expressed in hemocytes, gills, intestines and muscle but it was n...Continue Reading

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Citations

Oct 12, 2012·Applied Microbiology and Biotechnology·Julien VerdonJean-Marc Berjeaud
May 24, 2016·International Journal of Molecular Sciences·Annarita FalangaStefania Galdiero
Mar 25, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Tipachai VatanavicharnAnchalee Tassanakajon
Feb 11, 2010·Developmental and Comparative Immunology·Changkao MuLinsheng Song
Apr 8, 2010·Journal of Invertebrate Pathology·Suchao DonpudsaKenneth Söderhäll
Dec 1, 2010·Developmental and Comparative Immunology·Edward J NogaDavid Robinette
May 10, 2011·Fish & Shellfish Immunology·Changkao MuLinsheng Song

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