Molecular cloning and characterization of five opsin genes from the marine flatfish Atlantic halibut (Hippoglossus hippoglossus)

Visual Neuroscience
J V HelvikH C Seo

Abstract

Most molecular studies on the visual system in fish have been performed on freshwater teleosts such as goldfish and zebrafish where cones and rods appear simultaneously. Many marine fishes have long larval phase in the upper pelagic zone before transformation into a juvenile and a benthic life style. The retina at the larval stages consists of only single cone cells; later during metamorphosis double cones and rods develop. The flatfish Atlantic halibut (Hippoglossus hippoglossus) is a typical example of a marine species with such a two-step retina development. In this study, we have cloned five different opsins from Atlantic halibut larvae and juvenile retinas. Sequence comparisons with other opsins and phylogenetic analysis show that the five genes belong to the opsins of long-wavelength sensitive (L); middle-wavelength sensitive, M(Cone) and M(Rod); and short-wavelength sensitive, S(Blue) and S(Ultraviolet), respectively. In situ hybridization analysis reveals expression in double cone (L and M(Cone)), single cone (S(Blue) and S(Ultraviolet)), and rod (M(Rod)) types of photoreceptor cells in juvenile halibut retina. The visual system in Atlantic halibut seems therefore to have all four types of cone photoreceptors in additio...Continue Reading

Citations

Jun 5, 2004·Developmental and Comparative Immunology·Sigrun LangeBergljót Magnadóttir
Apr 16, 2010·Proceedings. Biological Sciences·Shelby TempleShaun P Collin
Jan 1, 2011·Marine Biology·K W VollsetH I Browman
Oct 29, 2015·Journal of Fish Biology·T Pavón-MuñozJ Francisco-Morcillo
Jan 12, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Rasmus Skern-MauritzenFrank Nilsen
Aug 18, 2004·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Shaun P Collin, Ann E O Trezise
Aug 27, 2010·Journal of Fish Biology·O Puebla
Oct 2, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Takeshi YamanomeAkiyoshi Takahashi
Jan 15, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Toshifumi Minamoto, Isamu Shimizu
Oct 19, 2006·The Journal of Comparative Neurology·W Ted AllisonCraig W Hawryshyn
Nov 18, 2017·The Journal of Experimental Biology·Ragnhild ValenJon Vidar Helvik
May 15, 2003·The Journal of Comparative Neurology·W Ted AllisonCraig W Hawryshyn
Feb 13, 2020·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Sara FrauJosé A Muñoz-Cueto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.