Molecular cloning and characterization of gene encoding for cytoplasmic Hsc70 from Pennisetum glaucum may play a protective role against abiotic stresses.

Molecular Genetics and Genomics : MGG
Palakolanu Sudhakar ReddyMalireddy K Reddy

Abstract

Molecular chaperones (Hsps) have been shown to facilitate protein folding or assembly under various developmental and adverse environmental conditions. The aim of this study was to unravel a possible role of heat-shock proteins in conferring abiotic stress tolerance to plants. We isolated a cDNA encoding a cytoplasmic Hsp70 (PgHsc70) from Pennisetum glaucum by screening heat-stress cDNA library. PgHsc70 cDNA encoding 649 amino acids represents all conserved signature motifs characteristic of Hsp70s. The predicted molecular model of PgHsc70 protein suggests that the N-terminus ATP-binding region is evolutionarily conserved, in comparison to C-terminus peptide-binding domains. A single intron in ATPase domain coding region of PgHsc70 exhibited a high degree of conservation with respect to its position and phasing among other plant Hsp70 genes. Recombinant PgHsc70 protein purified from E. coli possessed in vitro chaperone activity and protected PgHsc70 expressing bacteria from damage caused by heat and salinity stress. Nucleotide sequence analysis of 5' flanking promoter region of PgHsc70 gene revealed a potential heat-shock element (HSE) and other putative stress-responsive transcription factor binding sites. Positive correlation...Continue Reading

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Mar 13, 2008·Analytical Biochemistry·Palakolanu Sudhakar ReddyMalireddy K Reddy
Aug 5, 2008·Analytical Biochemistry·Palakolanu Sudhakar ReddyMalireddy K Reddy

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Citations

Apr 8, 2010·Extremophiles : Life Under Extreme Conditions·Shenghao LiuXiaohang Huang
Feb 9, 2017·Frontiers in Plant Science·Radha Shivhare, Charu Lata
Jun 10, 2011·Molecular Biology Reports·Narendra Singh YadavBhavanath Jha
Aug 24, 2017·Scientific Reports·Venkategowda RamegowdaMuthappa Senthil-Kumar

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