PMID: 2111018May 1, 1990Paper

Molecular cloning and characterization of GPA1, a G protein alpha subunit gene from Arabidopsis thaliana

Proceedings of the National Academy of Sciences of the United States of America
H MaE M Meyerowitz

Abstract

We have isolated a gene coding for a G protein alpha subunit from the flowering plant Arabidopsis thaliana. This gene, named GPA1, was isolated by using a DNA probe generated by polymerase chain reaction based on protein sequences from mammalian and yeast G protein alpha subunits. The sequences of genomic and cDNA clones indicate that GPA1 has 14 exons, and the deduced amino acid sequence shows that the GPA1 gene product (GP alpha 1) has 383 amino acid residues (44,582 Da). The GP alpha 1 protein exhibits similarity to all known G protein alpha subunits--36% of its amino acids are identical and 73% are similar (identical and conservative changes) to mammalian inhibitory guanine nucleotide-binding regulatory factor alpha subunits and transducins. Furthermore, the GP alpha 1 protein has all of the consensus regions for a GTP-binding protein. The GPA1-encoded mRNA of 1.55 kilobases is most abundant in vegetative plant tissues, as determined by RNA blot analysis. Restriction fragment length polymorphism mapping experiments show that GPA1 is approximately 1.2 centimorgans from the visible marker er on chromosome 2.

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R J SchmidtB Burr
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D C MedynskiH R Bourne
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M PupilloP N Devreotes
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M StrathmannM I Simon
Jan 27, 1989·Cell·E M Meyerowitz
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C ChangE M Meyerowitz
Mar 22, 1985·Science·D J Lipman, W R Pearson
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S IshiiI Pastan
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M NakafukuY Kaziro
Sep 30, 1988·Biochemical and Biophysical Research Communications·M JacobsP H Rubery
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H ItohK Suzuki
Jan 1, 1987·Annual Review of Biochemistry·A G Gilman
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T KozasaY Kaziro
Jul 12, 1988·Biochemistry·M A Lochrie, M I Simon
Oct 31, 1988·Biochemical and Biophysical Research Communications·W BlumE W Weiler
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·Y KaziroR Hernandez
Jan 1, 1987·Annual Review of Genetics·E M Meyerowitz
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V Volloch
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A G Hinnebusch

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Citations

Dec 1, 1994·Plant Molecular Biology·T D Elich, J Chory
Dec 1, 1994·Plant Molecular Biology·H Ma
May 20, 2008·Cellular and Molecular Life Sciences : CMLS·A V Smrcka
Jun 30, 1994·Journal of Biotechnology·U Römling, B Tümmler
Nov 1, 1991·Trends in Genetics : TIG·A Trewavas, S Gilroy
Aug 15, 1992·Gene·H MaE M Meyerowitz
Feb 1, 1991·Mechanisms of Development·K PalmeJ Schell
Jul 10, 2003·Molecular and Biochemical Parasitology·R P HirtT M Embley
Aug 17, 2002·Current Opinion in Plant Biology·Alan M Jones
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J A HadwigerR A Firtel
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K M WarpehaL S Kaufman
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T M WilkieM I Simon
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W Li, S M Assmann
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L C Romero, E Lam
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·W H Wu, S M Assmann
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·C A WeissH Ma
Apr 4, 2009·Journal of Experimental Botany·Haruko OkamotoMarc R Knight
Sep 11, 1991·Nucleic Acids Research·A SwaroopS M Weissman
Aug 5, 2009·Plant & Cell Physiology·Jianjun GuoJin-Gui Chen
Dec 31, 2009·Plant & Cell Physiology·Yuki IzawaYukimoto Iwasaki
Mar 29, 2013·Open Biology·Daisuke UranoAlan M Jones
Nov 4, 1998·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R Hooley

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