Molecular cloning and characterization of human WNT3

International Journal of Oncology
M Katoh

Abstract

Mouse Wnt-3 is a proto-oncogene, which is activated by mouse mammary tumor virus (MMTV). Human WNT3 cDNA fragment, previously isolated by another group, corresponds to a partial coding sequence. WNT3 cDNA, spanning the complete coding sequence, was isolated in this study. WNT3 encoded 355-amino-acid polypeptide with the N-terminal signal peptide and two N-linked glycosylation sites. WNT3 was most homologous to WNT3A (84.2% total amino-acid identity) among human WNTs. The WNT3 gene on the human chromosome 17q21 region consisted of five exons. WNT3 mRNAs were detected in fetal brain, adult brain, and testis by Northern blot analyses. WNT3 mRNA was relatively highly expressed in A549 cells (lung cancer) and MKN45 cells (gastric cancer) among 37 human cancer cell lines. WNT3 was significantly up-regulated in a case of primary breast cancer and in a case of primary rectal cancer among various types of human primary cancers. These results strongly suggest that WNT3 might play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT - beta-catenin - TCF signaling pathway, similar to mouse Wnt-3.

Citations

Dec 14, 2001·Biochemical and Biophysical Research Communications·M KatohK Shiokawa
Nov 11, 2011·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Nariyasu NakashimaHiroyasu Yokomise
Mar 22, 2005·The International Journal of Biochemistry & Cell Biology·Mark D SteelJane E Collins
Mar 26, 2010·Microbial Cell Factories·Ermenegilda ParrilliMaria Luisa Tutino
Jun 15, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ryan R DriskellJohn F Engelhardt

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