Molecular cloning and characterization of human tissue inhibitor of metalloproteinase 4.

The Journal of Biological Chemistry
J GreeneY E Shi

Abstract

The tissue inhibitors of metalloproteinases (TIMPs) constitute a family of proteins, of which three members have so far been described. Using the expressed sequence tag sequencing approach, we have identified a novel TIMP-related cDNA fragment and subsequently cloned a fourth human TIMP (TIMP-4) from a human heart cDNA library. The open reading frame encodes a 224-amino acid precursor including a 29-residue secretion signal. The predicted structure of the new protein shares 37% sequence identity with TIMP-1 and 51% identity with TIMP-2 and -3. The protein has a predicted isoelectric point of 7.34. The open reading frame-directed expression of TIMP-4 protein in MDA-MB-435 human breast cancer cells showed metalloproteinase inhibitory activity on reverse zymography. By Northern analysis, only the adult heart showed abundant TIMP-4 transcripts with a 1. 4-kilobase predominant transcript band; very low levels of the transcripts were detected in the kidney, placenta, colon, and testes, and no transcripts were detected in the liver, brain, lung, thymus, and spleen. This unique expression pattern suggests that TIMP-4 may function in a tissue-specific fashion in extracellular matrix homeostasis.

References

Nov 11, 1992·International Journal of Cancer. Journal International Du Cancer·H JoensuuS Toikkanen
Jul 22, 1991·Biochimica Et Biophysica Acta·W J Lennarz, W J Strittmatter
Dec 1, 1990·Anti-cancer Drugs·G SavaG Mestroni
Jun 1, 1990·The Biochemical Journal·R A WilliamsonR B Freedman
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G I GoldbergC S He
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D F CarmichaelG P Stricklin
Jul 1, 1994·DNA and Cell Biology·C G WildeJ J Seilhamer
Nov 1, 1994·International Journal of Cancer. Journal International Du Cancer·D W VisscherR Fridman
Jul 1, 1994·International Journal of Cancer. Journal International Du Cancer·M S NaylorF R Balkwill
Feb 15, 1994·International Journal of Cancer. Journal International Du Cancer·M HöyhtyäC M Liang
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·E J BernhardR J Muschel
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C WolfP Basset

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Citations

Nov 5, 1999·The Journal of Pathology·S Curran, G I Murray
Feb 14, 1998·International Journal of Cancer. Journal International Du Cancer·P ValenteA Albini
Sep 30, 1999·International Journal of Cancer. Journal International Du Cancer·H UenoY Okada
Feb 13, 2001·Journal of Cellular Biochemistry·C M TummalapalliS C Tyagi
Aug 30, 2001·Microscopy Research and Technique·M ShahinW Domschke
Sep 19, 2002·Molecular Reproduction and Development·Qing-Lei LiCheng Zhu
Feb 17, 2001·Biochemical and Biophysical Research Communications·S Hernandez-BarrantesR Fridman
Nov 24, 2004·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jens FielitzVera Regitz-Zagrosek
Mar 8, 2006·Histochemistry and Cell Biology·Ilpo KoskivirtaHannu Järveläinen
Nov 17, 2009·Cell and Tissue Research·Tim E Cawston, David A Young
Jul 23, 2005·Current Heart Failure Reports·Anne M Deschamps, Francis G Spinale
Jul 25, 2009·Current Treatment Options in Cardiovascular Medicine·Francis G Spinale, Nikole M Wilbur
Oct 3, 2003·Biochimica Et Biophysica Acta·Satoshi KubotaHaruhiko Toyohara
Mar 24, 2004·Critical Reviews in Oncology/hematology·Elise LambertEmmanuelle Petitfrère
Aug 26, 1998·The American Journal of Pathology·K LampertB Volk
Jun 8, 2002·Survey of Ophthalmology·Tina T L WongPeng T Khaw
Oct 24, 1998·Progress in Biophysics and Molecular Biology·N Borkakoti
Oct 6, 1997·Journal of Hepatology·E RoebS Matern
Jul 24, 2003·Journal of the American College of Cardiology·Christophe BoixelStéphane N Hatem
Feb 16, 2000·Journal of Dermatological Science·Y SugitaS Yamamoto
Sep 21, 2000·Pathophysiology : the Official Journal of the International Society for Pathophysiology·S C Tyagi
Mar 21, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·I Wu, M A Moses
Sep 28, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·L O NelimarkkaH T Järveläinen
Jul 7, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Y A DeClerck
Feb 27, 2003·Critical Reviews in Oncology/hematology·Ian ChauDavid Cunningham
May 1, 1998·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·S C Tyagi
Oct 28, 2010·Cardiology in the Young·Tain-Yen HsiaFrancis G Spinale
Nov 20, 1998·Breast Cancer Research and Treatment·C BenaudE W Thompson
Aug 13, 1999·Breast Cancer Research and Treatment·C LuparelloI Pucci-Minafra

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