Molecular cloning and characterization of IKKε gene from black carp Mylopharyngodon piceus

Fish & Shellfish Immunology
Yixiao QuHao Feng

Abstract

IKKε is an IκB kinase functioning in NF-κB signal pathway in the innate immune system of higher vertebrates. To exploit the function of IKKε of black carp (bcIKKε) in its antiviral innate immunity, the IKKε gene has been cloned from the RNA isolated from the spleen of black carp. The full-length cDNA of bcIKKε is 2537 bp, which encodes the peptide of 723 amino acids. bcIKKε contains a S-Tkc domain, a PKc domain and a UBL-TBK1-like domain and bcIKKε shares the highest amino acid sequence similarity with that of grass carp. bcIKKε was constitutively transcribed in the selected tissues of black carp including gill, kidney, heart, intestine, liver, muscle, skin and spleen; and the mRNA level of bcIKKε in these tissues varied right after SVCV or GCRV infection. bcIKKε had been well expressed in HEK293T cells and western blot assay determined that this fish kinase was around 80 KDa. The immunofluorescence assay of both NH3T3 cells and EPC cells demonstrated that bcIKKε was located in the cytosolic part of the cell. Report assay result showed that overexpression of bcIKKε in EPC cells activated the expression of both zebrafish IFN and EPC IFN. All our data suggest that bcIKKε is a novel fish kinase functioning in the innate antiviral ...Continue Reading

References

Apr 28, 2000·Nature·Y TojimaM Nakanishi
Jun 3, 2000·Annual Review of Immunology·M Karin, Y Ben-Neriah
May 9, 2001·Biochimica Et Biophysica Acta·R T Peters, T Maniatis
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
Apr 15, 2003·Nature Immunology·Katherine A FitzgeraldTom Maniatis
May 14, 2005·Fish & Shellfish Immunology·J H W M RomboutG Scapigliati
Jun 9, 2005·Fish & Shellfish Immunology·Børre Robertsen
Feb 18, 2006·Cell Death and Differentiation·H J KimA S Baldwin
Feb 25, 2006·Cell·Shizuo AkiraOsamu Takeuchi
Mar 3, 2007·Annual Review of Biochemistry·Myeong Sup Lee, Young-Joon Kim
Jan 6, 2009·Immunological Reviews·Mitsutoshi Yoneyama, Takashi Fujita
Jan 6, 2009·Immunological Reviews·Luigi FranchiGabriel Nuñez
Feb 28, 2009·International Immunology·Taro Kawai, Shizuo Akira
Mar 24, 2009·Annual Review of Immunology·Sivakumar Vallabhapurapu, Michael Karin
Jun 6, 2009·Annual Review of Biochemistry·Brian SkaugZhijian J Chen
Mar 31, 2010·Marine Biotechnology·Bergljot Magnadottir
Jan 12, 2011·Annual Review of Immunology·Jean-Laurent CasanovaLluis Quintana-Murci
Jul 26, 2011·Developmental and Comparative Immunology·Jun Zou, Christopher J Secombes
Jun 19, 2012·Annual Review of Genomics and Human Genetics·Thomas BoehmIsabell Hess
Mar 19, 2013·Annual Review of Biophysics·Johanna Napetschnig, Hao Wu
Jun 4, 2013·Trends in Microbiology·Claudia Gürtler, Andrew G Bowie
Jun 19, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yi WangPinghui Feng
Aug 29, 2013·Developmental and Comparative Immunology·Danilo Pietretti, Geert F Wiegertjes
Feb 7, 2015·Fish & Shellfish Immunology·Wei ZhouHao Feng
Jun 19, 2015·Immunological Reviews·Katsuhiro Sasaki, Kazuhiro Iwai
Jul 26, 2015·Fish & Shellfish Immunology·Zhilin HuangHao Feng

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