Molecular cloning and characterization of P113, a mouse SNF2/SWI2-related transcription factor

Gene
Q ZhangK H Kim

Abstract

A full-length cDNA encoding a 113-kDa transcription factor, named P113, was cloned from mouse preadipocyte line 30A5. P113 binds to a 7-bp consensus TNF-response element and a 30-bp fragment from mouse PAI-1 promoter (-88/-59). Sequence analysis indicates that the P113 is highly homologous to HIP116/HLTF (human) and RUSH-1alpha (rabbit). The sequence homology and the fact that P113 contains seven motifs conserved in many DNA-dependent helicases/ATPases indicate that it is a new member of the SNF2/SWI2 protein family. A cysteine-rich motif, called RING finger, was found close to the C-terminus of P113. The expression pattern of P113 mRNA in rat tissues is significantly different from that of HLTF in human tissues. Affinity-purified P113 has an ATPase activity that is activated by DNA in a sequence-specific manner. Using Northern blot analysis and the PAI-1 promoter/luciferase system, we demonstrated that P113 is a transcription factor that activates the transcription of the PAI-1 gene in 30A5 cells.

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Citations

Jul 20, 1999·Molecular and Cellular Endocrinology·J H DevineB S Chilton
Mar 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ildiko UnkLajos Haracska
Feb 7, 2001·Molecular and Cellular Biology·J R GuyonR E Kingston
Oct 17, 2015·Cellular and Molecular Life Sciences : CMLS·Ludovic DhontAlexandra Belayew
Feb 22, 2012·Growth Factors·Hiu Kiu, Sandra E Nicholson
Apr 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hichem LahouassaFlorence Margottin-Goguet
Jun 22, 2000·Biology of Reproduction·A RendonV H Lee
Jul 18, 2008·Biochemical Society Transactions·Beverly S Chilton, Aveline Hewetson
Feb 24, 2001·Annals of the New York Academy of Sciences·B S ChiltonM Mansharamani
Jun 18, 2017·DNA Repair·Natalia LugliThanos D Halazonetis

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