Molecular cloning and characterization of the 5'-flanking regulatory region of the carbonic anhydrase nacrein gene of the pearl oyster Pinctada fucata and its expression.

Biochemical Genetics
Tomoyuki MiyashitaRyosuke Takagi

Abstract

The carbonic anhydrase nacrein participates in the formation of the nacreous or prismatic layer of Pinctada fucata. We isolated a genomic clone containing the nacrein gene and cloned the 5'-flanking region. Within the 1336 bp 5' flanking region, we identified putative cis-acting elements, including the TATA box (TATAAAA) at -82 bp, and AP1 (-819 bp) and Oct-1 (-1244 bp) binding sites. In addition to the mantle, the nacrein gene is also expressed in the adductor muscle, liver, and foot. These results showed that nacrein not only takes part in the formation of the hard tissue but also might be involved in acid-base balance, ion transport, and maintenance of ionic concentration. In vitro transcription experiments showed that the addition of human c-jun activates transcription from the nacrein promoter. This is the first report of a promoter from a gene that controls the formation of the hard tissue of mollusk shells.

References

Dec 10, 1991·Biochimica Et Biophysica Acta·P Angel, M Karin
Jun 1, 1989·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R E Tashian
Jan 1, 1984·Annals of the New York Academy of Sciences·R Benesch
Jan 19, 1995·Nature·T Kerppola, T Curran
Mar 15, 1996·Biochimica Et Biophysica Acta·M Kakei, H Nakahara
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·H MiyamotoA Matsushiro
Jan 1, 1996·Annual Review of Physiology·R P Henry
Mar 1, 2000·Biochemical and Biophysical Research Communications·M KonoT Samata
May 31, 2001·Journal of Cellular Physiology·J P DavidR Baron
Oct 27, 2004·Journal of Enzyme Inhibition and Medicinal Chemistry·Silvia PastorekovaClaudiu T Supuran
Jan 13, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Zhenyan YuRongqing Zhang
Jan 8, 2008·Bioscience, Biotechnology, and Biochemistry·Tomoyuki MiyashitaNobuto Higashikubo
Sep 24, 2008·Chembiochem : a European Journal of Chemical Biology·Benjamin MarieFrédéric Marin

❮ Previous
Next ❯

Methods Mentioned

BETA
in vitro transcription
electrophoresis
in vitro transcriptions
Assay
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.