Molecular cloning and characterization of the ABA-specific glucosyltransferase gene from bean (Phaseolus vulgaris L.)

Journal of Plant Physiology
Sasikumar Arunachalam PalaniyandiSeung Hwan Yang

Abstract

Levels of the plant hormone abscisic acid (ABA) are maintained in homeostasis by a balance of its biosynthesis, catabolism and conjugation. The detailed molecular and signaling events leading to strict homeostasis are not completely understood in crop plants. In this study, we obtained cDNA of an ABA-inducible, ABA-specific UDP-glucosyltransferase (ABAGT) from the bean plant (Phaseolus vulgaris L.) involved in conjugation of a glucose residue to ABA to form inactive ABA-glucose ester (ABA-GE) to examine its role during development and abiotic stress in bean. The bacterially expressed PvABAGTase enzyme showed ABA-specific glucosylation activity in vitro. A higher level of the PvABAGT transcript was observed in mature leaves, mature flowers, roots, seed coats and embryos as well as upon rehydration following a period of dehydration. Overexpression of 35S::PvABAGT in Arabidopsis showed reduced sensitivity to ABA compared with WT. The transgenic plants showed a high level of ABA-GE without significant decrease in the level of ABA compared with the wild type (WT) during dehydration stress. Upon rehydration, the levels of ABA and phaseic acid (PA) decreased in the WT and the PvABAGT-overexpressing lines with high levels of ABA-GE onl...Continue Reading

References

Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·X Qin, J A Zeevaart
Jan 17, 2003·Plant Physiology·Inès M Brocard-GiffordRuth R Finkelstein
Apr 15, 2003·Plant Physiology·Steven H SchwartzJan A D Zeevaart
May 22, 2003·Trends in Plant Science·Eiji Nambara, Annie Marion-Poll
Sep 28, 2004·Journal of Experimental Botany·Elizabeth A Bray
May 3, 2005·Annual Review of Plant Biology·Eiji Nambara, Annie Marion-Poll
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·David M PriestDianna J Bowles
Jul 22, 2006·The Plant Journal : for Cell and Molecular Biology·Seung Hwan Yang, Jan A D Zeevaart

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