PMID: 2110241May 1, 1990Paper

Molecular cloning and characterization of the structural gene for the major iron-regulated protein expressed by Neisseria gonorrhoeae

The Journal of Experimental Medicine
S A BerishS A Morse

Abstract

This report describes the cloning and sequencing of the major iron-regulated protein (termed Fbp) of Neisseria gonorrhoeae strain F62. Attempts to identify recombinants expressing the Fbp using specific antibody proved unsuccessful. Therefore, an alternative cloning strategy using oligonucleotide probes derived from NH2-terminal and tryptic fragments of this protein was used to identify short fragments of the gene. Using this methodology, the gene encoding the precursor of Fbp was cloned on three separate overlapping fragments and sequenced, and the amino acid sequence was deduced. These data were unambiguously confirmed by the known NH2-terminal amino acid sequence and were supported by the sequences from tryptic fragments that lie outside of this region. Using oligonucleotide probes, we were unable to obtain clones encoding the potential regulatory region of this protein. Therefore, the technique of inverse polymerase chain reaction was used to amplify a fragment containing an additional 200 bp. This fragment was cloned and sequenced and found to contain a consensus ribosome binding site and potential -10 and -35 sequences. Hybridization analysis of genomic DNA from gonococcal strain F62 indicated that only a single copy of t...Continue Reading

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Citations

May 1, 1996·Trends in Microbiology·S D Gray-Owen, A B Schryvers
May 1, 1996·Trends in Microbiology·C A Genco, P J Desai
Jul 30, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C FerreirósJ A Gómez
Aug 11, 1990·Nucleic Acids Research·S A BerishS A Morse
Oct 1, 1991·Molecular Microbiology·A BihimaierC P Gibbs
Sep 1, 1992·Molecular Microbiology·S A BerishS A Morse
Feb 1, 1994·Molecular Microbiology·C E Thomas, P F Sparling
Dec 1, 1994·Molecular Microbiology·C N Cornelissen, P F Sparling
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Donna Perkins-BaldingIgor Stojiljkovic
Jan 1, 1992·Critical Reviews in Microbiology·B R OttoD M MacLaren
Aug 1, 1994·AORN Journal·J T Harden, N Girard
Sep 3, 2011·Environmental Microbiology·Brian M Hopkinson, Katherine A Barbeau
Jul 12, 2002·Canadian Journal of Microbiology·Lisa-Anne Agiato Foster
Oct 20, 1995·The Journal of Biological Chemistry·P AdhikariT A Mietzner
Jan 1, 1990·Medical Microbiology and Immunology·J P van Putten
Jan 1, 1991·Biology of Metals·A E Le Faou, S A Morse
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May 25, 1999·Infection and Immunity·S Sebastian, C A Genco
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Dec 1, 1995·Infection and Immunity·P J DesaiC A Genco
Nov 1, 1993·Infection and Immunity·S A BerishS A Morse

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