PMID: 3320956Dec 10, 1987Paper

Molecular cloning and characterization of the gene encoding the DNA methyltransferase, M.CviBIII, from Chlorella virus NC-1A

Nucleic Acids Research
K E NarvaJ L van Etten

Abstract

The gene encoding the DNA methyltransferase, M.CviBIII, from Chlorella virus NC-1A was cloned and expressed in E. coli plasmid pUC8. Plasmid (pNC-1A.14.8) encoded M.CviBIII methylates adenine in TCGA sequences both in vivo in E. coli and in vitro. Transposon Tn5 mutagenesis localized the M.CviBIII functional domain to a 1.5 kbp region of pNC-1A.14.8 and also indicated that a virus promoter directs transcription of the gene in E. coli. The 2.1 kbp insert containing the M.CviBIII gene was sequenced and a single open reading frame of 1131 bp was identified within the domain determined by Tn5 mutagenesis. When the M.CviBIII gene was fused in-frame with the 19 amino-terminal codons of lacZ a 45 kD polypeptide was identified in maxicells as predicted by the DNA sequence. The M.CviBIII gene was not essential for virus replication since a virus M.CviBIII deletion mutant also replicated in Chlorella.

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Citations

Dec 25, 1988·Gene·J L Van EttenK E Narva
Dec 25, 1988·Gene·J PósfaiR J Roberts
Oct 20, 2005·Journal of Bioscience and Bioengineering·T YamadaS Hiramatsu
Feb 11, 1989·Nucleic Acids Research·D KupperA Kiss
Apr 11, 1989·Nucleic Acids Research·J PósfaiR J Roberts
Apr 25, 1990·Nucleic Acids Research·R J Roberts
May 25, 1991·Nucleic Acids Research·G G Wilson
Feb 25, 1992·Nucleic Acids Research·H ItoI Kato
Jun 17, 1998·Biological Chemistry·M NelsonJ L Van Etten
Mar 20, 1989·Journal of Molecular Biology·R Lauster
May 1, 1990·Virology·D E BurbankJ L Van Etten
Nov 5, 1999·Annual Review of Microbiology·J L Van Etten, R H Meints
Sep 1, 1988·Applied and Environmental Microbiology·Y P ZhangJ L Van Etten
Dec 1, 1991·Microbiological Reviews·J L Van EttenR H Meints

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