PMID: 8605256Feb 7, 1996Paper

Molecular cloning and characterization of the major allergen Myr p II from the venom of the jumper ant Myrmecia pilosula: Myr p I and Myr p II share a common protein leader sequence

Biochimica Et Biophysica Acta
M D StreetB A Baldo

Abstract

A major allergen Myr p II of the Australian jumper ant Myrmecia pilosula has been cloned, immunocharacterized and nucleotide sequenced. An open reading frame of 225 bases was identified and found to encode a deduced amino acid sequence of 75 residues which contained a typical hydrophobic peptide leader sequence. Expressed fusion proteins of Myr p II in both phage and plasmid vectors bind high levels of ant venom-specific IgE and the expressed clones are recognised by 35% of ant venom-allergic individuals. IgE antibodies that recognise the expressed clone have been shown to recognise IgE-binding bands in blots of native venom after separation by SDS-PAGE. The amino acid sequence of Myr p II shares close structural homology with the other major jumper ant allergen Myr p I, differing by only three amino acids in the first 47 residues of both sequences. However, N-terminal analysis of IgE-binding bands derived from Tricine-SDS-PAGE gel blots indicates that both Myr p I and Myr p II undergo extensive post-translational proteolytic processing to unique peptides of 45 and 27 residues, respectively.

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Citations

Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·Noel W DaviesSimon G A Brown
Aug 8, 2002·Toxicon : Official Journal of the International Society on Toxinology·Forbes McGain, Kenneth D Winkel
Dec 22, 2004·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Graham O Solley
Apr 7, 2009·Journal of Proteomics·Dirk C de GraafBart Devreese
Dec 8, 2015·Molecular Immunology·Hathairat SrisongAndreas L Lopata
Aug 22, 2006·Toxicon : Official Journal of the International Society on Toxinology·James Tibballs
Dec 27, 2005·Toxicon : Official Journal of the International Society on Toxinology·Michael D WieseRobert J Heddle
Dec 3, 2014·Toxicon : Official Journal of the International Society on Toxinology·Samira R AiliGraham M Nicholson
Mar 1, 2015·Toxicon : Official Journal of the International Society on Toxinology·Troy WanandyMichael D Wiese
Jul 6, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Troy WanandyMichael D Wiese
Apr 20, 2004·Australian Veterinary Journal·L A AbrahamS A Holloway
Sep 15, 2020·Drugs·Connie Kang, Yahiya Y Syed
Dec 19, 2019·BMC Pharmacology & Toxicology·Ibrahim M AlhazzaAshraf M A Mashaly
Feb 12, 2002·The Medical Journal of Australia·S G BrownB A Baldo

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