PMID: 9529048Apr 7, 1998Paper

Molecular cloning and characterization of the genes coding for the highly immunogenic cluster of 90-kilodalton envelope proteins from the Chlamydia psittaci subtype that causes abortion in sheep

Infection and Immunity
D LongbottomA J Herring

Abstract

Proteins present in the outer membrane of chlamydiae that are involved in mucosal epithelial cell infection must clearly be identified and characterized if we are to understand and modify the pathogenic mechanisms utilized by these organisms. We have identified and isolated a family of four genes encoding putative outer membrane proteins (POMPs), a group of proteins of approximately 90 kDa present in the outer membrane of the subtype of Chlamydia psittaci that causes ovine enzootic abortion (strain S26/3). These proteins, although minor components, are major immunogens, as shown by the immunoblotting of chlamydial outer membrane complexes with postabortion sheep sera, and are therefore potential diagnostic and/or protective antigen candidates. Immunoblotting of the expressed amino- and carboxy-terminal halves of one of the POMPs with postabortion sheep sera showed that the major humoral immune response appeared to be directed solely against the amino-terminal half. This result, in combination with the positive immunofluorescence staining of S26/3-infected cells using POMP-specific (specific to the amino-terminal half of the proteins) monoclonal antibodies, suggests the probable surface localization of the POMPs and, more specif...Continue Reading

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Citations

May 20, 2015·Pathogens and Disease·Carole Kebbi-BeghdadiGilbert Greub
Oct 2, 2015·Mediators of Inflammation·Tamsyn DerrickMartin J Holland
Aug 24, 2016·The Journal of Biological Chemistry·Sören E T LuczakJohannes H Hegemann
Mar 20, 2001·Infection and Immunity·R J TanzerT P Hatch
Feb 8, 2002·Microbiology·Erika A LindquistRichard S Stephens
Jul 2, 2008·Infection and Immunity·Ine Jorgensen, Raphael H Valdivia
Feb 26, 2020·Critical Reviews in Microbiology·Arlieke GitselsDaisy Vanrompay
Mar 29, 2001·Journal of Bacteriology·R J Tanzer, T P Hatch
Aug 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Francis O EkoJoseph U Igietseme
Nov 7, 2000·Microbiology·P M BavoilD M Ojcius
Jun 6, 2000·The Journal of Infectious Diseases·G ChristiansenS Birkelund
Jun 10, 2005·Veterinary Research Communications·G MasalaS Tola
Jul 5, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Nieves OrtegaMaría C Gallego
Sep 19, 2000·Infection and Immunity·D D RockeyR S Stephens
Jun 17, 2003·Journal of Medical Microbiology·David Longbottom
Aug 10, 2007·Australian Veterinary Journal·L M E McCauleyC G V Ainsworth
Oct 5, 2001·FEMS Microbiology Letters·A S PedersenS Birkelund
Jun 2, 2001·Journal of the Royal Society of Medicine·G EntricanD Longbottom

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