Molecular cloning and characterization of two genes encoding dihydroflavonol-4-reductase from Populus trichocarpa.

PloS One
Yan HuangKeming Luo

Abstract

Dihydroflavonol 4-reductase (DFR, EC 1.1.1.219) is a rate-limited enzyme in the biosynthesis of anthocyanins and condensed tannins (proanthocyanidins) that catalyzes the reduction of dihydroflavonols to leucoanthocyanins. In this study, two full-length transcripts encoding for PtrDFR1 and PtrDFR2 were isolated from Populus trichocarpa. Sequence alignment of the two PtrDFRs with other known DFRs reveals the homology of these genes. The expression profile of PtrDFRs was investigated in various tissues of P. trichocarpa. To determine their functions, two PtrDFRs were overexpressed in tobacco (Nicotiana tabacum) via Agrobacterium-mediated transformation. The associated color change in the flowers was observed in all 35S:PtrDFR1 lines, but not in 35S:PtrDFR2 lines. Compared to the wild-type control, a significantly higher accumulation of anthocyanins was detected in transgenic plants harboring the PtrDFR1. Furthermore, overexpressing PtrDFR1 in Chinese white poplar (P. tomentosa Carr.) resulted in a higher accumulation of both anthocyanins and condensed tannins, whereas constitutively expressing PtrDFR2 only improved condensed tannin accumulation, indicating the potential regulation of condensed tannins by PtrDFR2 in the biosyntheti...Continue Reading

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Citations

Sep 1, 2015·Frontiers in Plant Science·Konstantinos KoudounasPolydefkis Hatzopoulos
Oct 8, 2016·Frontiers in Plant Science·Marçal SolerJacqueline Grima-Pettenati
Jan 20, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min HeXuehao Chen
Apr 4, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yunting ZhangHaoru Tang
May 18, 2018·FEMS Yeast Research·Michael EichenbergerMichael Naesby
Mar 21, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dylan O'Neill RothenbergLingyun Zhang
Jan 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Caibi ZhouLingyun Zhang
Oct 9, 2020·International Journal of Molecular Sciences·Grzegorz Chrzanowski
Apr 4, 2021·International Journal of Molecular Sciences·Helena Gómez-MartínezMaria L Badenes

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Datasets Mentioned

BETA
AY147903
AAA32783
AAC25960
AAD26204
CAA53578
CAA91924
CAA78930
AA12736
BAA22072
BAA74700

Methods Mentioned

BETA
reverse transcription-PCR
PCR
transgenic
electrophoresis
transgenics

Software Mentioned

DNAstar
DNAMAN

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