Molecular cloning and characterization of tropomyosin, a major allergen of Chironomus kiiensis, a dominant species of nonbiting midges in Korea

Clinical and Diagnostic Laboratory Immunology
Kyoung Yong JeongTai-Soon Yong

Abstract

Chironomids are widely and abundantly distributed in the vicinity of standing waters. Larvae of Chironomus and some other genera are known to contain hemoglobins, which have been described as a major allergen, and the adults that have no hemoglobins also have been reported to contain allergens. In this study, we tried to establish the role of chironomid allergy and characterize the allergen of Chironomus kiiensis adults. Skin tests using C. kiiensis adult extracts were performed on patients with allergic symptoms. A cDNA library of C. kiiensis adults was screened with C. kiiensis immune mouse sera to identify allergens, and results were confirmed using skin test-positive human sera. Recombinant allergen was expressed in Escherichia coli and purified by affinity chromatography using nickel-nitrilotriacetic acid agarose to investigate its allergenic properties. Out of 275 allergic patients 14.2% showed a positive reaction to C. kiiensis adult crude extracts in the skin test. The tropomyosin was cloned by immunoscreening and expressed in Escherichia coli. C. kiiensis tropomyosin has a high homology at the amino acid level with tropomyosins which were previously known to be allergens in various arthropods (Periplaneta americana, 86...Continue Reading

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Citations

May 10, 2011·Revue des maladies respiratoires·J-C Bessot, G Pauli
Aug 12, 2015·ZooKeys·Gizelle AmoraVanderly Andrade-Souza
May 14, 2017·Molecular Nutrition & Food Research·Henrike C H P BroekmanKitty C M Verhoeckx
Jul 17, 2019·Current Protein & Peptide Science·Kyoung Yong Jeong, Jung-Won Park
Jul 10, 2004·Clinical and Diagnostic Laboratory Immunology·Kyoung Yong JeongTai-Soon Yong
Nov 2, 2012·Allergy, Asthma & Immunology Research·Kyoung Yong JeongChein-Soo Hong
Apr 14, 2011·Yonsei Medical Journal·Kyoung Yong JeongJung-Won Park
Jul 25, 2019·Allergy, Asthma & Immunology Research·Myung Hee YiTai Soon Yong
Nov 4, 2009·The Korean Journal of Parasitology·Tai-Soon Yong, Kyoung Yong Jeong
Mar 25, 2017·Molecular Medicine Reports·Kyoung Yong JeongJung-Won Park
Jul 1, 2005·Physiological Reviews·Scott L Hooper, Jeffrey B Thuma
Jul 20, 2017·The American Journal of Tropical Medicine and Hygiene·Shumaila NazShelley F Walton
Aug 25, 2021·Molecular Biology Reports·Nur Farah Hani AzemiHaidr Msahir Ateshan

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