Molecular cloning and characterization of autophagy-related gene TmATG8 in Listeria-invaded hemocytes of Tenebrio molitor

Developmental and Comparative Immunology
Hamisi TindwaYeon Soo Han

Abstract

Macroautophagy (hereinafter called autophagy) is a highly regulated process used by eukaryotic cells to digest portions of the cytoplasm that remodels and recycles nutrients and disposes of unwanted cytoplasmic constituents. Currently 36 autophagy-related genes (ATG) and their homologs have been characterized in yeast and higher eukaryotes, including insects. In the present study, we identified and functionally characterized the immune function of an ATG8 homolog in a coleopteran insect, Tenebrio molitor (TmATG8). The cDNA of TmATG8 comprises of an ORF of 363 bp that encodes a protein of 120 amino acid residues. TmATG8 transcripts are detected in all the developmental stages analyzed. TmAtg8 protein contains a highly conserved C-terminal glycine residue (Gly116) and shows high amino acid sequence identity (98%) to its Tribolium castaneum homolog, TcAtg8. Loss of function of TmATG8 by RNAi led to a significant increase in the mortality rates of T. molitor larvae against Listeria monocytogenes. Unlike dsEGFP-treated control larvae, TmATG8-silenced larvae failed to turn-on autophagy in hemocytes after injection with L. monocytogenes. These data suggest that TmATG8 play a role in mediating autophagy-based clearance of Listeria in T...Continue Reading

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Citations

Nov 15, 2018·Journal of Fungi·Patrícia Canteri de SouzaRicardo Sergio Almeida
Aug 24, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Gianluca Tettamanti, Morena Casartelli
Feb 16, 2020·International Journal of Molecular Sciences·Tariku Tesfaye EdosaYeon Soo Han
Apr 26, 2020·International Journal of Molecular Sciences·Chang Eun KimYong Hun Jo
Jun 18, 2021·Frontiers in Immunology·Yong Hun JoYeon Soo Han

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