Molecular cloning and characterization of Fasciola gigantica thioredoxin-glutathione reductase

Parasitology Research
Narin ChangklungmoaKulathida Chaithirayanon

Abstract

The Fasciola gigantica thioredoxin-glutathione reductase (FgTGR) gene is a fusion between thioredoxin reductase (TR) and a glutaredoxin (Grx) gene. FgTGR was cloned by polymerase chain reaction (PCR) from adult complementary DNA (cDNA), and its sequences showed two isoforms, i.e., the cytosolic and mitochondrial FgTGR. Cytosolic FgTGR (cytFgTGR) was composed of 2370 bp, and its peptide had no signal sequence and hence was not a secreted protein. Mitochondrial FgTGR (mitFgTGR) was composed of 2506 bp with a signal peptide of 43 amino acids; therefore, it was a secreted protein. The putative cytFgTGR and mitFgTGR peptides comprised of 598 and 641 amino acids, respectively, with a molecular weight of 65.8 kDa for cytFgTGR and mitFgTGR, with a conserved sequence (CPYC) of TR, and ACUG and CVNVGC of Grx domains. The recombinant FgTGR (rFgTGR) was expressed in Escherichia coli BL21 (DE3) and used for production for a polyclonal antibody in rabbits (anti-rFgTGR). The FgTGR protein expression, estimated by indirect ELISA using the rabbit anti-rFgTGR as probe, showed high levels of expression in eggs, and 2- and 4-week-old juveniles and adults. The rFgTGR exhibited specific activities in the 5,5'-dithiobis (2-nitro-benzoic acid) (DTNB) ...Continue Reading

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Jul 3, 2013·Experimental Parasitology·Pathanin ChantreePrasert Sobhon
Mar 7, 2014·Experimental Parasitology·Kant SangpairojKulathida Chaithirayanon
Apr 11, 2014·Parasitology Research·Narin ChangklungmoaKulathida Chaithirayanon

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Citations

May 31, 2016·Parasitology International·Timir TripathiBanchob Sripa
Jun 25, 2016·Acta Tropica·Wipaphorn JaikuaNarin Changklungmoa
Feb 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alberto Guevara-FloresIrene Patricia Del Arenal
Aug 27, 2018·Parasitology Research·Narin ChangklungmoaPornanan Kueakhai
Sep 16, 2019·International Journal of Biological Macromolecules·Nikhilesh Joardar, Santi P Sinha Babu
Apr 20, 2021·Parasitology International·Pornanan KueakhaiKrai Meemon

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