Molecular cloning and chromosome mapping of rat phospholipase D genes, Pld1a, Pld1b and Pld2

Cytogenetics and Cell Genetics
S NakashimaY Nozawa

Abstract

We have previously obtained three partial rat phospholipase D (PLD) cDNA fragments by a reverse transcriptase-polymerase chain reaction (RT-PCR) method using degenerate primers based on two conserved amino acid sequences in PLDs of human and yeast. The entire coding regions of these genes were isolated and sequenced. The longest clone, Pld1a encodes a 1075 amino acid (aa) protein that was highly similar (89% identity) to human PLD1a, especially in four conserved regions present in other PLDs. The nucleotide sequence of the second clone was identical to that of Pld1a except that the clone lacked 114 nucleotides corresponding to 38 aa in the middle. A shorter alternatively spliced form of human PLD1 (PLD1b) lacking the corresponding 38 aa was also identified. Therefore, the second clone (Pld1b) was considered to correspond to the rat counterpart of human PLD1b. The third clone, Pld2 encoding 933 aa was smaller than that of Pld1 and its aa identity to rat Pld1 was 56%. However, it contains four conserved regions and aa sequences of these regions are homologous to those of rat Pld1 and human PLD1. Its entire aa sequence was very similar (96% identity) to the recently cloned mouse PLD, Pld2. Chromosome locations of the Pld1a, Pld1b ...Continue Reading

Citations

Nov 24, 1999·Journal of Cellular Physiology·T C Gibbs, K E Meier
Jan 15, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·A Gómez-MuñozU P Steinbrecher
Jul 4, 2002·The Journal of Eukaryotic Microbiology·S WangY Nozawa
Jun 8, 1999·Chemistry and Physics of Lipids·S Nakashima, Y Nozawa
Jul 30, 1999·Biochimica Et Biophysica Acta·K OhguchiY Nozawa
Jan 11, 2000·Clinical Microbiology Reviews·M A Ghannoum

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