Molecular cloning and comparative analysis of a y-type inactive HMW glutenin subunit gene from cultivated emmer wheat (Triticum dicoccum L.)

Hereditas
Minmin SunFriedrich J Zeller

Abstract

Cultivated emmer (Triticum dicoccum, 2n = 4x = 28, AABB) is closely related to bread wheat and possesses extensive allelic variations in high molecular weight glutenin subunit (HMW-GS) composition. These alleles may be an important genetic resource for wheat quality improvement. To isolate and clone HMW-GS genes from cultivated emmer, two pairs of allele-specific (AS) PCR primers were designed to amplify the coding sequence of y-type HMW-GS genes and their upstream sequences, respectively. The results showed that single bands of strong amplification were obtained through AS-PCR of genomic DNA from emmer. After cloning and sequencing the complete sequence of coding and 5'-flanking regions of a y-type subunit gene at Glu-A1 locus was obtained. Nucleotide and deduced amino acid sequences analysis showed that this gene possessed a similar structure as the previously reported Ay gene from common wheat, and is hence designated as Ay1d. The distinct feature of the Ay1d gene is that its coding region contains four stop codons and its upstream region has a 85-bp deletion in the same position of the Ay gene, which are probably responsible for the silencing of y-type subunit genes at Glu-A1 locus. Phylogenetic analysis of HMW glutenin sub...Continue Reading

References

Oct 11, 1985·Nucleic Acids Research·R D ThompsonN P Harberd
Sep 1, 1987·Molecular & General Genetics : MGG·N P HarberdR D Thompson
Feb 1, 1993·Genome Génome / Conseil National De Recherches Canada·J DvorákP Resta
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Oct 1, 1987·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·V Vallega, J G Waines

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Citations

Sep 22, 2009·Genome Génome / Conseil National De Recherches Canada·O D Anderson
Oct 19, 2006·Yi chuan xue bao = Acta genetica Sinica·Zu-Jun YangZheng-Long Ren
Aug 29, 2006·Genome Génome / Conseil National De Recherches Canada·Yanzhen ZhangFriedrich J Zeller
Dec 31, 2020·International Journal of Molecular Sciences·Yi LiDong Yang

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