Molecular cloning and differential expression pattern of two structural variants of the crustacean hyperglycemic hormone family from the mud crab Scylla olivacea

General and Comparative Endocrinology
Kuo-Wei TsaiChi-Ying Lee

Abstract

Two full-length cDNA sequences encoding a crustacean hyperglycemic hormone (CHH) precursor were cloned from tissues of the mud crab Scylla olivacea. Sco-CHH (S. olivacea CHH) was cloned from eyestalk ganglia, whereas Sco-CHH-L (S. olivacea CHH-like peptide) was cloned from extra-eyestalk tissues (pericardial organ and thoracic ganglia). Each conceptually translated precursor is expected to be processed into a signal peptide, a CHH precursor-related peptide (CPRP), and a mature CHH or CHH-like peptide. The two precursors are identical in amino acid sequence through the 40th residue of the mature peptide, but different from each other substantially in the C-terminus. Both CHH variants contain the six highly conserved cysteine residues characteristic of the CHH family peptides, and share higher sequence identities with other brachyuran CHH sequences than with those of other taxonomic groups. As determined by reverse transcription-polymerase chain reaction (RT-PCR), the transcripts of Sco-CHH and Sco-CHH-L were present in eyestalk ganglia and several extra-eyestalk tissues (the thoracic ganglia, pericardial organ, brain, circumesophageal connectives, and gut). Sco-CHH was the predominant form in eyestalk ganglia, while Sco-CHH-L wa...Continue Reading

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Citations

Jan 25, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Su-Hua WuChi-Ying Lee
Dec 8, 2011·General and Comparative Endocrinology·Simon George WebsterHeinrich Dircksen
May 9, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ye-Qing QianWei-Jun Yang
Jun 1, 2011·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Paola Pérez-PolancoUbaldo García
Oct 30, 2020·Frontiers in Endocrinology·Hsiang-Yin ChenChi-Ying Lee

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