PMID: 2116013Aug 1, 1990Paper

Molecular cloning and DNA sequence analysis of a diphtheria tox iron-dependent regulatory element (dtxR) from Corynebacterium diphtheriae

Proceedings of the National Academy of Sciences of the United States of America
J BoydJ R Murphy

Abstract

Although the structural gene for diphtheria toxin, tox, is carried by a family of closely related corynebacteriophages, the regulation of tox expression is controlled, to a large extent, by its bacterial host Corynebacterium diphtheriae. Optimal yields of tox gene products are obtained only when iron becomes the growth-rate-limiting substrate. Previous studies suggest that regulation of tox expression is mediated through an iron-binding aporepressor. To facilitate molecular cloning of the tox regulatory element from genomic libraries of C. diphtheriae, we constructed a tox promoter/operator (toxPO)-lacZ transcriptional fusion in Escherichia coli strain DH5 alpha. We report the molecular cloning and nucleic acid sequence of a diphtheria tox iron-dependent regulatory element, dtxR, and demonstrate that expression of beta-galactosidase from the toxPO-lacZ fusion is regulated by dtxR-encoded protein in an iron-sensitive manner. In addition, we show that expression of the toxPO-lacZ fusion is not affected by the E. coli iron-regulatory protein Fur and that the dtxR protein does not inhibit expression of fur-regulated outer-membrane proteins.

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Citations

Jan 1, 1991·Biology of Metals·E Griffiths
May 24, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Michaël L CartronSimon C Andrews
Oct 9, 2002·Journal of Molecular Biology·Jennifer L LavrrarMark A McIntosh
Jun 19, 2002·Journal of Microbiological Methods·John F Love, John R Murphy
Sep 27, 2000·FEMS Microbiology Letters·C A Boland, W G Meijer
Dec 21, 2006·Biochemistry·Misha V GolynskiySeth M Cohen
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M P SchmittR K Holmes
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·X Tao, J R Murphy
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·X Tao, J R Murphy
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·X TaoJ R Murphy
Feb 5, 2000·The Journal of Infectious Diseases·R K Holmes
Mar 25, 1992·Nucleic Acids Research
Jul 17, 2007·Applied and Environmental Microbiology·Kelly S BenderJudy D Wall
Oct 6, 2005·Applied and Environmental Microbiology·Tzu-Chiao ChaoStefan Weidner
Jul 16, 2002·Infection and Immunity·Patrick L Wagner, Matthew K Waldor
Feb 27, 2007·Journal of Bacteriology·Carey A Kunkle, Michael P Schmitt
Apr 16, 2013·Journal of Bacteriology·Courtni E AllenMichael P Schmitt
Jan 11, 2011·Journal of Bacteriology·Julia FrunzkeMichael Bott
Mar 20, 2003·Journal of Bacteriology·Jennifer L Lavrrar, Mark A McIntosh
Apr 6, 2006·Journal of Bacteriology·Julia Wennerhold, Michael Bott

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