Molecular cloning and expression analysis of rat Rgs12 and Rgs14

Biochemical and Biophysical Research Communications
B E SnowD P Siderovski

Abstract

We report the cloning of two novel rat regulators of G-protein signaling (RGS) cDNAs using a degenerate PCR strategy. The rRgs12 and rRgs14 cDNAs encode predicted polypeptides of 1387 and 544 amino acids, respectively. We have also identified the human orthologue of rRgs12 by alignment of cosmid sequences in the database which map the human RGS12 gene to chromosome 4p16.3. Furthermore, we identified human ESTs with high homology to rRgs14 which map to human chromosome 5qter. Northern blot analysis indicates that rRgs14 is expressed at high levels in brain, lung, and spleen, whereas rRgs12 is expressed at high levels in brain and lung and lower levels in testis, heart, and spleen. Analysis of the predicted rRGS12 and rRGS14 polypeptides indicates that they are closely related and possess regions of homology outside of the conserved RGS domain. We have also identified conserved regions in RGS12 which are similar to protein domains found in mouse rhophilin and coiled-coil proteins suggesting possible interactions with ras-like G-proteins.

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Citations

Sep 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sarah Emerson LeeJohn R Hepler
Jun 11, 2004·Annual Review of Biochemistry·Francis S WillardDavid P Siderovski
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·L De VriesM G Farquhar
Jun 2, 2007·Biological & Pharmaceutical Bulletin·Mitsunori IkedaYohsuke Minatogawa
Mar 26, 2009·PloS One·Francis S WillardDavid P Siderovski
Jun 14, 2005·International Journal of Biological Sciences·David P Siderovski, Francis S Willard
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·J MaoD Wu
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·B E SnowD P Siderovski
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·L De VriesM G Farquhar
Dec 15, 2015·Molecular Pharmacology·Kyle J GerberJohn R Hepler
May 13, 1999·The Journal of Biological Chemistry·M A Diversé-PierluissiL De Vries
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