Molecular cloning and expression analysis of the sucrose transporter gene family from Theobroma cacao L

Fupeng LiJianxiong Lai


In this study, we performed cloning and expression analysis of six putative sucrose transporter genes, designated TcSUT1, TcSUT2, TcSUT3, TcSUT4, TcSUT5 and TcSUT6, from the cacao genotype 'TAS-R8'. The combination of cDNA and genomic DNA sequences revealed that the cacao SUT genes contained exon numbers ranging from 1 to 14. The average molecular mass of all six deduced proteins was approximately 56 kDa (range 52 to 66 kDa). All six proteins were predicted to exhibit typical features of sucrose transporters with 12 trans-membrane spanning domains. Phylogenetic analysis revealed that TcSUT2 and TcSUT4 belonged to Group 2 SUT and Group 4 SUT, respectively, and the other TcSUT proteins were belonging to Group 1 SUT. Real-time PCR was conducted to investigate the expression pattern of each member of the SUT family in cacao. Our experiment showed that TcSUT1 was expressed dominantly in pods and that, TcSUT3 and TcSUT4 were highly expressed in both pods and in bark with phloem. Within pods, TcSUT1 and TcSUT4 were expressed more in the seed coat and seed from the pod enlargement stage to the ripening stage. TcSUT5 expression sharply increased to its highest expression level in the seed coat during the ripening stage. Expression patte...Continue Reading


Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·J M Lu, D R Bush
Jul 19, 2000·The Plant Cell·L BarkerWolf B Frommer
Jan 5, 2002·Progress in Lipid Research·Stephen Rawsthorne
May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Elke RoscheJohn W Patrick
Apr 2, 2003·Plant & Cell Physiology·Naohiro AokiRobert T Furbank
Sep 21, 2004·Annual Review of Plant Biology·Sylvie LalondeWolf B Frommer
May 3, 2005·Annual Review of Plant Biology·Hans WeberUlrich Wobus
Oct 26, 2005·Photosynthesis Research·Norbert RollandJacques Joyard
Dec 22, 2005·The Plant Journal : for Cell and Molecular Biology·Aleksandra HackelChristina Kühn
Apr 1, 1992·Plant Physiology·V C Pence
Aug 26, 2006·Plant, Cell & Environment·Anke ReindersJohn M Ward
Apr 17, 2007·FEBS Letters·Norbert Sauer
Aug 31, 2007·Journal of Experimental Botany·Graham N ScofieldRobert T Furbank
Jun 13, 2009·Plant & Cell Physiology·Alexandre BerthierNathalie Noiraud-Romy
Mar 23, 2010·Current Opinion in Plant Biology·Christina Kühn, Christopher P L Grof
Dec 28, 2010·Nature Genetics·Xavier ArgoutClaire Lanaud
Jan 26, 2011·The Plant Journal : for Cell and Molecular Biology·Raja S PayyavulaScott A Harding
Nov 19, 2011·Genetics and Molecular Research : GMR·T T PinheiroA Figueira
Nov 2, 2013·The Plant Cell·Nancy A Eckardt

Related Concepts

SLC26A7 gene
Seed Maturation
Real-Time Polymerase Chain Reaction
Plant Bark
Cacao Plant

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.