Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa ) sucrose transporter gene family

Plant Diversity
Yuanyuan LiuYongping Yang

Abstract

In higher plants, sugars (mainly sucrose) are produced by photosynthetically assimilated carbon in mesophyll cells of leaves and translocated to heterotrophic organs to ensure plant growth and development. Sucrose transporters, or sucrose carriers (SUCs), play an important role in the long-distance transportation of sucrose from source organs to sink organs, thereby affecting crop yield and quality. The identification, characterization, and molecular function analysis of sucrose transporter genes have been reported for monocot and dicot plants. However, no relevant study has been reported on sucrose transporter genes in Brassica rapa var. rapa, a cruciferous root crop used mainly as vegetables and fodder. We identified and cloned 12 sucrose transporter genes from turnips, named BrrSUC1.1 to BrrSUC6.2 according to the SUC gene sequences of B. rapa pekinensis. We constructed a phylogenetic tree and analyzed conserved motifs for all 12 sucrose transporter genes identified. Real-time quantitative polymerase chain reaction was conducted to understand the expression levels of SUC genes in different tissues and developmental phases of the turnip. These findings add to our understanding of the genetics and physiology of sugar transport...Continue Reading

Datasets Mentioned

BETA
AB071809
AB091674
AAM13408
AAM13409
AAM13410
AAS91375
AAT51689

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

FigTree1
- Needle
MAGA6
MEME
ExPASy
Oligo7
BLAST BLASTP )
ProtParam
Gene Structure Display Servers
ClustalW

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