Molecular cloning and expression in different microbes of the DNA encoding Pseudomonas putida U phenylacetyl-CoA ligase. Use of this gene to improve the rate of benzylpenicillin biosynthesis in Penicillium chrysogenum.

The Journal of Biological Chemistry
B MiñambresJ M Luengo

Abstract

The gene encoding phenylacetyl-CoA ligase (pcl), the first enzyme of the pathway involved in the aerobic catabolism of phenylacetic acid in Pseudomonas putida U, has been cloned, sequenced, and expressed in two different microbes. In both, the primary structure of the protein was studied, and after genetic manipulation, different recombinant proteins were analyzed. The pcl gene, which was isolated from P. putida U by mutagenesis with the transposon Tn5, encodes a 48-kDa protein corresponding to the phenylacetyl-CoA ligase previously purified by us (Martínez-Blanco, H., Reglero, A. Rodríguez-Aparicio, L. B., and Luengo, J. M. (1990) J. Biol. Chem. 265, 7084-7090). Expression of the pcl gene in Escherichia coli leads to the appearance of this enzymatic activity, and cloning and expression of a 10.5-kb DNA fragment containing this gene confer this bacterium with the ability to grow in chemically defined medium containing phenylacetic acid as the sole carbon source. The appearance of phenylacetyl-CoA ligase activity in all of the strains of the fungus Penicillium chrysogenum transformed with a construction bearing this gene was directly related to a significant increase in the quantities of benzylpenicillin accumulated in the broth...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1992·DNA Sequence : the Journal of DNA Sequencing and Mapping·P R BirchP Broda
Dec 1, 1992·Applied and Environmental Microbiology·A SchmitzR Eichenlaub
Jan 1, 1992·Journal of Biochemistry·T AbeT Yamamoto
Aug 1, 1992·Journal of Bacteriology·J R Driscoll, H W Taber
Oct 25, 1990·Nucleic Acids Research·Y ZhaoS K Dube
Jan 1, 1987·Gene·F SánchezM A Peñalva
Nov 15, 1983·Journal of Molecular Biology·A M FrischaufN Murray
Sep 1, 1994·Molecular Microbiology·K EichlerM A Mandrand-Berthelot
Aug 2, 1994·Molecular & General Genetics : MGG·V GarreS Torres-Martínez
Apr 1, 1993·Applied and Environmental Microbiology·M Fernández-ValverdeJ M Luengo
Nov 1, 1995·The Journal of Antibiotics·J M Luengo

❮ Previous
Next ❯

Citations

Sep 5, 2009·Applied Microbiology and Biotechnology·Bin CaoKai-Chee Loh
Jun 1, 2011·Folia Microbiologica·Zhou-Liang YuHua Zhang
Nov 27, 1998·Trends in Biotechnology·M A PeñalvaG Turner
May 17, 2003·Metabolic Engineering·Jette Thykaer, Jens Nielsen
Nov 23, 2007·Natural Product Reports·J Stefan RokemJens Nielsen
May 15, 2012·Applied and Environmental Microbiology·Wael Ismail, Johannes Gescher
Feb 5, 2013·Applied and Environmental Microbiology·Rita A LuuRebecca E Parales
Aug 6, 2004·Applied and Environmental Microbiology·Belén GarcíaJosé M Luengo
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·E R OliveraJ M Luengo
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·E DíazJ L García
Jul 7, 2007·Biochemical and Biophysical Research Communications·Fu-Qiang WangJian-Gong He
Sep 25, 1998·The Journal of Biological Chemistry·A FerrándezE Díaz
May 4, 2005·The Journal of Biological Chemistry·Elsa Arias-BarrauJosé M Luengo
Oct 7, 2008·The Biochemical Journal·Martijn J KoetsierDick B Janssen

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.