Molecular cloning and expression in Escherichia coli of bleomycin-resistance gene from a methicillin-resistant Staphylococcus aureus and its association with IS431 mec

Applied Microbiology and Biotechnology
M Z BhuiyanM Sugiyama

Abstract

A gene that confers bleomycin resistance was cloned from the chromosomal DNA of methicillin-resistant Staphylococcus aureus (MRSA) B-26 into the plasmid pUC18. It is of chromosomal origin rather than plasmid and exists in the chromosome making a cluster with the kanamycin-resistance gene. We found that the nucleotide sequence of the bleomycin-resistance gene from the chromosome of MRSA B-26 is identical to that from a staphylococcal plasmid, pUB110. The partial sequence of IS431mec was also found upstream from the DNA fragment containing the bleomycin- and kanamycin-resistance genes.

Citations

Feb 18, 2009·Chembiochem : a European Journal of Chemical Biology·Isao MiyazakiHiroyuki Osada
Feb 7, 2001·Applied and Environmental Microbiology·H Y Kim, Y J Choi

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