PMID: 11604168Oct 18, 2001Paper

Molecular cloning and expression of a cDNA encoding the luciferase from the firefly, Pyrocoelia rufa

Journal of Biotechnology
K S LeeB R Jin

Abstract

To clone a cDNA encoding the luciferase of the firefly, Pyrocoelia rufa, we have constructed a cDNA library and isolated the luciferase gene using PCR with gene specific primers. Sequence analysis of the cDNA encoding the luciferase of P. rufa revealed that the 1647 bp cDNA has an open reading frame of 548 amino acid residues. The deduced amino acid sequences of the luciferase gene of P. rufa showed 98.9% homology to that of P. miyako. Phylogenetic analysis further confirmed the deduced amino acid sequences of the P. rufa luciferase gene belonged to the same subfamily, Lampyrinae. Southern blot analysis suggested possible presence of the P. rufa luciferase gene as a single copy and Northern blot analysis confirmed light organ-specific expression pattern at the transcriptional level. The cDNA encoding the luciferase of P. rufa was expressed as a 69 kDa band in baculovirus-infected insect cells and the recombinant baculovirus-infected cell extracts emitted luminescence in the luciferase activity assay.

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Citations

Jul 3, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Ewan M CampbellAlan S Bowman
Jul 2, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yong Soo ChoiByung Rae Jin
Feb 6, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yong Soo ChoiByung Rae Jin
Jun 20, 2014·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Joshua B BenoitGeoffrey M Attardo
Nov 4, 2004·Biochemical and Biophysical Research Communications·Bagher Said AlipourShahrokh Kazempour Osaloo
Oct 28, 2009·Cellular and Molecular Life Sciences : CMLS·Satoshi Inouye
Nov 5, 2016·Photochemistry and Photobiology·Yasuo MitaniYoshihiro Ohmiya
Apr 1, 2009·Expert Opinion on Drug Discovery·Hideto Hoshino
Jul 1, 2017·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Manabu Bessho-UeharaYuichi Oba
Feb 12, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·V R VivianiY Ohmiya
Dec 22, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·A J Silva NetoV R Viviani
Apr 12, 2019·Photochemistry and Photobiology·Gui-Chun LiuXue-Yan Li
Aug 5, 2021·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Jin-Wu HeXue-Yan Li

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