Molecular cloning and expression of a new class of ortho-diphenol-O-methyltransferases induced in tobacco (Nicotiana tabacum L.) leaves by infection or elicitor treatment

Plant Physiology
L PellegriniM Legrand

Abstract

In tobacco (Nicotiana tabacum L. cv Samsun NN), three distinct enzymes account for ortho-diphenol-O-methyltransferase (OMT) activity. OMT I is the major enzyme of healthy leaves, whereas enzymes OMT II and III are preferentially induced during the hypersensitive reaction to tobacco mosaic virus (TMV). Using an anti-OMT III antiserum, we isolated a partial OMT III cDNA clone by immunoscreening an expression library made from mRNA of TMV-infected tobacco leaves. Using this OMT III clone as a probe, we isolated a full-length clone with a deduced amino acid sequence encompassing all of the sequences obtained by Edman degradation of both purified proteins II and III. Thus, OMT II and III of tobacco are likely to be encoded by the same genes and to arise from different posttranslational modifications. Sequence analysis showed that this OMT clone represents a new class of OMT enzymes (class II) with a low level of similarity (53-58%) to OMTs cloned previously from other dicotyledonous plants. Southern analysis indicated that a small family of class II OMT genes inherited from ancestors related to Nicotiana sylvestris and Nicotiana tomentosiformis occurs in the tobacco genome. RNA blot analysis demonstrated that class II OMT genes, unl...Continue Reading

References

Dec 1, 1992·Plant Molecular Biology·P CollazoJ Rigau
Feb 1, 1988·Immunology Letters·M H Van Regenmortel, G Daney de Marcillac
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M A Saghai-MaroofR W Allard

❮ Previous
Next ❯

Citations

Dec 1, 1994·Plant Molecular Biology·L GregersenD B Collinge
Feb 24, 2001·Phytochemistry·S FrickT M Kutchan
Jan 5, 2002·Phytochemistry·Gudrun SchröderJoachim Schröder
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·E A MaherC J Lamb
Apr 3, 2010·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Alagu ManickaveluYasunari Ogihara
Dec 18, 2008·Journal of Experimental Botany·Sandrine MathieuDenise M Tieman
Jan 28, 2005·Molecular Plant-microbe Interactions : MPMI·Hajime TsunezukaKo Shimamoto
Nov 13, 2003·Plant Physiology·Jeroen RaesWout Boerjan
Apr 3, 2013·Plant Physiology·Tariq A Akhtar, Eran Pichersky
Oct 1, 1994·The Plant Cell·Z H YeJ E Varner
Dec 7, 2007·Genome Génome / Conseil National De Recherches Canada·Kevin C LamSelvadurai Dayanandan
Dec 9, 2014·Plant Signaling & Behavior·Dinesh GuptaChhandak Basu
Apr 21, 2006·The Plant Journal : for Cell and Molecular Biology·Heather CoinerJoachim Schröder
Nov 9, 2007·The Plant Journal : for Cell and Molecular Biology·Britta RohdeUlrich Matern
Nov 23, 2011·The Plant Journal : for Cell and Molecular Biology·Melissa H MageroyHarry J Klee
Jan 1, 1997·DNA Sequence : the Journal of DNA Sequencing and Mapping·J E LeeM Löbler
Jul 1, 2010·Comprehensive Reviews in Food Science and Food Safety·Rupinder SinghUpendra N Dwivedi
Feb 1, 1995·The New Phytologist·A M BoudetJ Grima-Pettenati
Jul 23, 1999·Archives of Biochemistry and Biophysics·J Wang, E Pichersky

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.