Molecular cloning and expression of a novel human beta-Gal-3-O-sulfotransferase that acts preferentially on N-acetyllactosamine in N- and O-glycans

The Journal of Biological Chemistry
A SuzukiM Fukuda

Abstract

A novel cDNA-encoding galactose 3-O-sulfotransferase was cloned by screening the expressed sequence tag data base using the previously cloned cDNA encoding a galactosyl ceramide 3-O-sulfotransferase, which we term Gal3ST-1. The newly isolated cDNA encodes a novel 3-O-sulfotransferase, termed Gal3ST-3, that acts exclusively on N-acetyllactosamine present in N-glycans and core2-branched O-glycans. These conclusions were confirmed by analyzing CD43 chimeric proteins in Chinese hamster ovary cells expressing core2 beta1,6-N-acetylglucosaminyltransferase. The acceptor specificity of Gal3ST-3 contrasts with that of the recently cloned galactose 3-O-sulfotransferase (Honke, K., Tsuda, M., Koyota, S., Wada, Y., Iida-Tanaka, N., Ishizuka, I., Nakayama, J., and Taniguchi, N. (2001) J. Biol. Chem. 276, 267-274), which we term Gal3ST-2 in the present study because the latter enzyme can also act on core1 O-glycan and type 1 oligosaccharides, Galbeta1-->3GlcNAc. Moreover, Gal3ST-3 but not Gal3ST-2 can act on Galbeta1-->4(sulfo-->6)GlcNAc, indicating that disulfated sulfo-->3Galbeta1-->4(sulfo-->6) GlcNAc-->R may be formed by Gal3ST-3 in combination with GlcNAc 6-O-sulfotransferase. Although both Gal3ST-2 and Gal3ST-3 do not act on galactosyl...Continue Reading

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Citations

Oct 9, 2002·Medicinal Research Reviews·Koichi Honke, Naoyuki Taniguchi
Jun 1, 2005·Biochemical and Biophysical Research Communications·Bi-Zhi ShiXing-Zhong Wu
Nov 13, 2004·Drug Discovery Today·Stefan HemmerichVirginia L Rath
Mar 7, 2007·Organic & Biomolecular Chemistry·Hai Yu, Xi Chen
Jun 23, 2009·Glycobiology·Kyoko ShidaYasuhide Miyamoto
Apr 12, 2013·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Koichi Honke
May 31, 2002·Japanese Journal of Cancer Research : Gann·Akira SekoKatsuko Yamashita
Oct 10, 2002·Endocrine Reviews·Charles A Strott
Jul 30, 2019·Frontiers in Genetics·Priscila S N de OliveiraLuciana C A Regitano
Feb 6, 2020·The Journal of Biological Chemistry·Candice QuinWesley F Zandberg
Oct 5, 2001·The Journal of Biological Chemistry·M FukudaM N Fukuda
Aug 16, 2001·The Journal of Biological Chemistry·N IkedaK Honke
Aug 11, 2010·Biochemistry·Chun-Yi ZhangXing Zhong Wu

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