Molecular cloning and expression of a novel human trans-Golgi network glycoprotein, TGN51, that contains multiple tyrosine-containing motifs.

The Journal of Biological Chemistry
R KainM Fukuda

Abstract

Previously, it has been shown that glycoproteins with approximately 130-kDa molecular mass react with antisera from patients with renal vasculitis (Kain, R., Matsui, K., Exner, M., Binder, S., Schaffner, G., Sommer, E. M., and Kerjaschki, D. (1995) J. Exp. Med. 181, 585-597). To search for a molecule that reacts with the antibodies, we screened a lambdagt11 human placental cDNA library. Two of the isolated clones were found to encode a putative counterpart of the rodent trans-Golgi network (TGN) glycoprotein 38, hTGN46, which has the tyrosine containing motif YQRL shared by mouse and rat TGN38. Moreover, reverse transcription-polymerase chain reaction analysis of hTGN46 transcripts and genomic analysis of a cDNA deposited as an expressed sequence tag in dbEST Data Base revealed that additional cDNAs exist that are produced by alternate usage of 3'-splice sites of intron III. Alternative splicing results in frame shifts and leads to novel larger translation products with one (for hTGN48) or two (for hTGN51) additional tyrosine-containing motifs. hTGN51 expressed in Chinese hamster ovary cells were localized to the trans-Golgi network, overlapping with beta-1,4-galactosyltransferase even after mutating the tyrosine-containing mot...Continue Reading

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Citations

Oct 7, 2008·Nature Medicine·Renate KainDontscho Kerjaschki
Jan 18, 2011·The Journal of Biological Chemistry·Zhiqiu ManHye-Won Shin
Jun 16, 2001·Molecular Biology of the Cell·J Rohrer, R Kornfeld
Dec 22, 1998·Molecular Membrane Biology·B J ReavesG Banting
Dec 27, 2011·Toxicology and Applied Pharmacology·François MarceauGuillaume Morissette
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaodong WangTomas Mustelin
Jan 19, 2013·Cell Structure and Function·Hiroyuki TakatsuKazuhisa Nakayama
Sep 1, 2005·Journal of Cell Science·Hye-Won ShinKazuhisa Nakayama

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