PMID: 9440134Jan 24, 1998Paper

Molecular cloning and expression of a rat prostaglandin E2 receptor of the EP2 subtype

Prostaglandins
K NemotoL G Raisz

Abstract

Prostaglandin E2 (PGE2) is a potent local mediator of cell growth and differentiation in various tissues. The receptors for PGE2 have been classified into four pharmacological subtypes, EP1, EP2, EP3, and EP4, based on the responses to selective agonists and antagonists. We have cloned a functional cDNA for the rat EP2 receptor subtype from a rat lung cDNA library. The rat EP2 receptor cDNA encodes 357 amino acid residues having high homology with the human and mouse EP2 receptors and containing seven putative transmembrane domains. In COS-7 cells transfected with rat EP2 cDNA, specific [3H]PGE2 binding was found with a dissociation constant of 14.9 nM, and this binding was inhibited by unlabeled PGE2 and PGE2 alpha. PGE2 and butaprost, an EP2 selective agonist, were effective in increasing the cAMP level in the COS-7 cell transfectants. Northern blot and RT-PCR analysis showed widespread distribution of the EP2 receptor in various tissues. Higher EP2 expression was found in fetal long bones and calvariae than in adult by RT-PCR and in situ hybridization, suggesting a role for this receptor in rapidly growing skeletal tissue.

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Citations

Jul 20, 1999·Prostaglandins & Other Lipid Mediators·T A HibbsT A Owen
Mar 14, 2002·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Marchessini Casibang, Terry W Moody
Oct 12, 2001·The Journal of Steroid Biochemistry and Molecular Biology·V L SylviaZ Schwartz
Jan 22, 2003·Journal of Neuroendocrinology·T NagataI Shibuya
Feb 2, 2000·Current Opinion in Nephrology and Hypertension·M D Breyer, R M Breyer
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·R M BreyerM D Breyer
Feb 22, 2001·Annual Review of Physiology·M D Breyer, R M Breyer
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Oct 9, 2007·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Chooryung J ChungKunimichi Soma
Mar 15, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Maxime A GallantArtur J de Brum-Fernandes
Jan 14, 2004·Clinical Physiology and Functional Imaging·Daina LasaitienePeter Friberg
Jun 13, 2012·Journal of Tissue Engineering and Regenerative Medicine·X Z YanX F Walboomers
Apr 6, 2004·Prostaglandins, Leukotrienes, and Essential Fatty Acids·I FortierA J de Brum-Fernandes
Aug 5, 2006·Seminars in Arthritis and Rheumatism·Stephen B Miller
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Jun 19, 2013·Biochemical and Biophysical Research Communications·Kyoshiro TsugeYukihiko Sugimoto
May 4, 2001·Prostaglandins, Leukotrienes, and Essential Fatty Acids·P SarrazinA J de Brum-Fernandes
Jul 6, 2010·Prostaglandins & Other Lipid Mediators·Cui YangGeorge Pak-Heng Leung
May 12, 2009·Expert Opinion on Investigational Drugs·Simon GrahamEleftherios Tsiridis
Jun 29, 2017·Pharmacological Reviews·Anna CooperAndrea J Vernall
Jun 19, 2013·Pharmacological Reviews·Utako YokoyamaYoshihiro Ishikawa
Jan 10, 2018·Expert Opinion on Drug Safety·Ippokratis Pountos, Peter V Giannoudis
Dec 28, 2019·Journal of Clinical Medicine·Ippokratis PountosPeter V Giannoudis
Oct 14, 2004·Clinical Orthopaedics and Related Research·Mary B Goldring, Francis Berenbaum

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