Molecular cloning and expression of rat liver aminopeptidase B.

The Journal of Biological Chemistry
Kayoko M FukasawaM Harada

Abstract

We isolated, by immunological screening of a Uni-ZAP XR cDNA library constructed from rat liver mRNAs, a cDNA clone with 2212 base pairs encoding aminopeptidase B (EC 3.4.11.6). The open reading frame encodes a 649-amino acid protein with a theoretical molecular mass of 72,545 Da and bears the consensus sequence of the zinc metalloexopeptidases, indicating that the enzyme belongs to this family, which includes aminopeptidase A, aminopeptidase N, and leukotriene-A4 hydrolase. Escherichia coli SOLR cells infected with the pBluescript phagemid excised from the Uni-ZAP XR vector containing the aminopeptidase B cDNA had a high L-arginyl-beta-naphthylamidase activity. The recombinant protein was purified to homogeneity from the recombinant E. coli extracts. The enzyme had Cl--dependent aminopeptidase activity specifically restricted to the Arg and Lys derivatives and contained 1 mol of zinc per mol of the enzyme.

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Citations

Aug 7, 1999·Clinical Reviews in Allergy & Immunology·J Z Haeggström
Aug 2, 2003·International Journal of Food Microbiology·Tomás BolumarFidel Toldrá
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Jun 12, 2003·The Journal of Biological Chemistry·Toshihiro TaniokaMasafumi Tsujimoto
Nov 5, 2014·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Junzo Hirose

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