PMID: 11900981Mar 20, 2002Paper

Molecular cloning and expression of the chromatin insulator protein CTCF in Xenopus laevis

Mechanisms of Development
L J BurkeR Renkawitz

Abstract

The zinc finger protein CTCF has been shown to mediate multiple functions connected to gene repression. Transcriptional inhibition as well as enhancer blocking and chromatin insulation are documented for CTCF in men, mice and chickens. Additionally, hCTCF has been linked to epigenetics and disease. In line with these basic cellular functions, CTCF has been found to be expressed in every cell type and adult tissue tested and has thus been deemed an ubiquitous protein. Here, we report the identification of the CTCF homologue from Xenopus and the analysis of the spatio-temporal expression of xCTCF during embryogenesis. Within the DNA binding domain, xCTCF is virtually identical to other identified vertebrate CTCF proteins. Homology also extends to other conserved regions that are important for CTCF function. Although xCTCF mRNA is present during all stages of early Xenopus development, a remarkable increase in expression is observed in neuronal tissues. Early in development, xCTCF is highly expressed in the neural plate and later in the neural tube and developing brain. By tailbud stage, elevated expression is also seen in the developing sensory organs of the head. This is the first detailed description of the expression pattern o...Continue Reading

References

Aug 30, 2001·Trends in Genetics : TIG·R OhlssonV Lobanenkov

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Citations

Feb 26, 2004·Experimental Cell Research·Ru ZhangRainer Renkawitz
Mar 26, 2003·The Journal of Steroid Biochemistry and Molecular Biology·L J BurkeR Renkawitz
Aug 23, 2002·Seminars in Cancer Biology·Elena M KlenovaVictor V Lobanenkov
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Peter HegerThomas Wiehe
Dec 6, 2002·Nucleic Acids Research·Pierre-Antoine Defossez, Eric Gilson
Sep 5, 2008·PLoS Genetics·Timothy A HoreJennifer A Marshall Graves
Dec 19, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Rolf OhlssonElena Klenova
Mar 21, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Meghan S AdamsMarianne Bronner-Fraser
Jun 23, 2007·Experimental Cell Research·Tie Li, Luo Lu
Oct 10, 2014·Biology of Reproduction·Maurício M FrancoRebecca J Oakey
Apr 21, 2004·The Journal of Biological Chemistry·Tie LiLuo Lu
Jan 29, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M Valeria Canto-SolerRuben Adler
Jul 12, 2017·Scientific Reports·Mitsutaka KadotaShigehiro Kuraku
Apr 1, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Miho MiyakawaTsutomu Kinoshita

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