Molecular cloning and expression of the large subunit of ADP-glucose pyrophosphorylase from barley (Hordeum vulgare) leaves

Gene
K EimertL A Kleczkowski

Abstract

A cDNA clone, blpl14, corresponding to the large subunit of ADP-glucose pyrophosphorylase (AGPase), has been isolated from a cDNA library prepared from leaves of barley (Hordeum vulgare L.). An open reading frame encodes a protein of 503 aa, with a calculated molecular weight of 54815. The derived aa sequence contains a putative transit peptide sequence, required for targeting to plastids, and has a highly conserved positioning of critical Lys residues that are believed to be involved in effector binding. The derived aa sequence shows 97% identity with the corresponding protein from wheat, but only 36% identity with AGPase from E. coli. The blpl14 gene is expressed predominantly in leaves and to a lesser degree in seed endosperm, but not roots, of barley.

References

Jan 1, 1992·Annual Review of Cell Biology·M Kozak
Feb 22, 1991·Cell·N Proudfoot
Mar 10, 1995·Molecular & General Genetics : MGG·U La CognataB Müller-Röber
Nov 1, 1992·Plant Physiology·P VillandL A Kleczkowski
Sep 1, 1987·Plant Physiology·M K MorellJ Preiss
Feb 1, 1994·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·A KilianL A Kleczkowski

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Citations

Sep 7, 2001·Plant Science : an International Journal of Experimental Plant Biology·B Y ChenT Gianfagna
Mar 27, 2009·Plant Physiology·Volodymyr V RadchukWinfriede Weschke
Jul 15, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Gautam Saripalli, Pushpendra Kumar Gupta

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