Molecular cloning and functional characterization of porcine cyclic GMP-AMP synthase

Molecular Immunology
Jiang WangGuoyu Yang

Abstract

Cyclic GMP-AMP synthase (cGAS), which belongs to the nucleotidyltransferase family, recognizes cytosolic DNA and induces the type I interferon (IFN) pathway through the synthesis of the second messenger cGAMP. In this study, porcine cGAS (p-cGAS) was identified and its tissue distribution, subcellular localization, and functions in innate immunity were characterized. The coding sequence of p-cGAS is 1494 bp long, encodes 497 amino acids, and is most similar (74%) to Bos taurus cGAS. p-cGAS mRNA is abundant in the spleen, duodenum, jejunum, and ileum. The subcellular distribution of p-cGAS is not only in the cytosol, but also on the endoplasmic reticulum (ER) membrane. The overexpression of wild-type p-cGAS in porcine kidney epithelial cells, but not its catalytically inactive mutants, induced IFN-β expression, which was dependent on STING and IRF3. However, the downregulation of p-cGAS by RNA interference markedly reduced IFN-β expression after pseudorabies virus (PRV) infection or poly(dA:dT) transfection. These results demonstrate that p-cGAS is an important DNA sensor, required for IFN-β activation.

References

Jan 16, 2003·Immunology Letters·Shizuo Akira, Hiroaki Hemmi
Aug 6, 2003·The Journal of Experimental Medicine·Jennifer LundAkiko Iwasaki
Apr 29, 2004·European Journal of Immunology·Ling LinStanford L Peng
Sep 24, 2004·Developmental Biology·Ryuichi YamadaNaoki Takahashi
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Lisa E PomeranzChristoph J Hengartner
Jan 18, 2006·Immunity·Daniel B Stetson, Ruslan Medzhitov
Jun 19, 2007·Nature Immunology·Eicke LatzDouglas T Golenbock
Mar 28, 2009·Journal of Lipid Research·Jiang WangBao-Liang Song
May 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wenxiang SunZhengfan Jiang
Oct 5, 2010·Nature Immunology·Leonie UnterholznerAndrew G Bowie
Mar 8, 2012·Science Signaling·Yasuo Tanaka, Zhijian J Chen
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Aug 3, 2014·Developmental and Comparative Immunology·Xinyu ZhuLiurong Fang

❮ Previous
Next ❯

Citations

May 18, 2018·Animal Science Journal = Nihon Chikusan Gakkaihō·Hiroki ShinkaiHirohide Uenishi
Apr 15, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhi-Fei LiuJian-Zhong Shao
Jul 8, 2020·Chembiochem : a European Journal of Chemical Biology·Katrin RosenthalStephan Lütz
Dec 28, 2019·International Journal of Molecular Sciences·Jascha RolfKatrin Rosenthal
Jan 22, 2021·Virus Research·Xiaoyong ChenGuangzhi Tong
May 29, 2021·Science China. Life Sciences·Guoli LiBeibei Chu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.