Molecular cloning and functional characterization of a mouse bradykinin B1 receptor gene

Biochemical and Biophysical Research Communications
J B PesqueroM Bader

Abstract

The gene encoding a putative mouse bradykinin B1 receptor was cloned from a genomic library by low stringency screening. Analysis of two isolated clones revealed a region which contains an open reading frame uninterrupted by introns and encodes a 334 amino acid protein, which exhibits seven potential transmembrane domains and is 68% identical to the human and rabbit bradykinin B1 receptors. Lipopolysaccharide-treatment induces B1 receptor transcripts in the heart, liver, and lung. Stable expression of the coding region in COS-7 cells resulted in high levels of binding sites for the specific B1 ligand des-ARG10 kallidin (Kd = 1.3 nM; Bmax = 51 fmol/mg protein). The rank order of affinity of the receptor for the agonists and antagonists was: des-Arg9BKdes-Arg9Leu8BKdes- Arg10kallidin > Hoe-140=bradykinin. Functional coupling of the cloned receptor was demonstrated by the dose-dependent effects of des-Arg(9)BK on the extracellular acidification rate in stably transfected COS-7 cells. This effect was not produced by bradykinin and could be blocked by the B1 antagonist des-Arg9Leu8BK.

Citations

Apr 27, 1999·British Journal of Pharmacology·M TrevisaniP Geppetti
Nov 9, 2002·Autonomic & Autacoid Pharmacology·K M Ellis, J R Fozard
Mar 22, 2001·Biological Chemistry·D RegoliF Gobeil
Mar 22, 2001·Biological Chemistry·R C AraújoM Bader
Feb 25, 2006·Biological Chemistry·João B Pesquero, Michael Bader
Apr 12, 2006·Biological Chemistry·Ronaldo C AraújoJoão B Pesquero
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·J B PesqueroM Bader
Jun 28, 2000·Proceedings of the National Academy of Sciences of the United States of America·D Regoli
Aug 17, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·K H Hsieh, J M Stewart
Apr 3, 2001·European Journal of Pharmacology·M M CamposJ B Calixto
Jan 9, 1999·European Journal of Pharmacology·T S SalehJ B Calixto
Feb 2, 1999·Clinical Reviews in Allergy & Immunology·F Marceau, D R Bachvarov
May 5, 2000·Fundamental & Clinical Pharmacology·C LagneuxC Ribuot
Apr 28, 2009·Experimental Brain Research·Kazue MizumuraYasuko Kozaki
Dec 1, 1998·British Journal of Pharmacology·C LagneuxC Ribuot
May 2, 2003·British Journal of Pharmacology·Jadranka EricPierre Sirois
Jun 21, 2016·European Journal of Pharmacology·Mohamed M El-KadyAhmed A Soliman
Jan 7, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J B Pesquero, M Bader
Jul 10, 2002·The Journal of Peptide Research : Official Journal of the American Peptide Society·C SchumannS Reissmann
May 14, 1998·British Journal of Pharmacology·L LiH Vapaatalo
May 29, 2003·British Journal of Pharmacology·Danielle R SantosGlória E P Souza
May 15, 1998·The American Journal of Physiology·M E Marin CastañoJ L Bascands
Mar 22, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Jean-Philippe FortinFrancois Marceau
Mar 29, 2013·Journal of Clinical Periodontology·Thais Oliveira Gonçalves-ZilloJoão Bosco Pesquero
Jun 28, 2016·Journal of Veterinary Pharmacology and Therapeutics·B A DuclosS Kamerling
Jun 11, 1999·Biochemical and Biophysical Research Communications·H TsukagoshiM Mori
Jul 28, 1999·European Journal of Pharmacology·C JonesP McIntyre
Nov 8, 2001·European Journal of Pharmacology·R CoutureF Cloutier
Apr 22, 1999·Trends in Pharmacological Sciences·A Ahluwalia, M Perretti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.