Molecular cloning and functional characterization of the oxytocin receptor from a rat pancreatic cell line (RINm5F)

Neuropeptides
Y J JengM S Soloff

Abstract

Oxytocin (OT) and vasopressin (AVP) stimulate insulin and glucagon release from the pancreas, and evoke insulin secretion from the rat insulinoma cell line, RINm5F. To determine which AVP/OT receptor subtype is expressed in RINm5F cells, we used PCR with degenerate primers to two transmembrane domains of the AVP (V1a, V1b (or V3), V2) and OT receptors (OTRs). The single PCR fragment identified was used to obtain a full length cDNA from a RINm5F cDNA library. Comparison of the deduced amino acid sequence of this clone with uterine OTR sequences from several species (human, sheep, bovine) and to the pig kidney epithelial cell (LLC-PK1) OTR reveals a very high degree of homology. After the RIN cell OTR cDNA was stably transfected into CHO cells (CHO-OTR), the cell membranes bound iodinated oxytocin antagonist with an apparent Kd comparable to that of RIN cell membranes and those from other OT target cells. Comparison of the ligand specificities of CHO-OTR and RIN cells membranes showed that the relative Ki values of a series of OT analogues were approximately equivalent in both preparations. The rank order of apparent Ki values also corresponded to published values for the rat myometrium, where OT elicits intracellular calcium tra...Continue Reading

References

Sep 16, 1975·Biochemical and Biophysical Research Communications·M S Soloff
Apr 9, 1992·Nature·T KimuraH Okayama
May 15, 1991·The Biochemical Journal·Z Y GaoJ C Henquin
Sep 21, 1990·European Journal of Pharmacology·A el AljG Germain
Apr 25, 1990·European Journal of Pharmacology·D J PettiboneJ A Totaro
Feb 1, 1990·Cell Calcium·R Y Tsien, A T Harootunian
Nov 1, 1989·Cell Calcium·P F Blackmore, G Augert
Feb 1, 1987·The Journal of Clinical Investigation·J PhilippeJ F Habener
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Jul 1, 1983·Analytical Biochemistry·R F BrunsT A Pugsley
Jun 19, 1995·American Journal of Medical Genetics·S MicheliniD Goldman
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·S J LolaitM J Brownstein
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·F RozenH H Zingg
Apr 1, 1995·Endocrinology·N L OstrowskiS J Lolait
Dec 1, 1995·DNA and Cell Biology·R BathgateR Ivell
Dec 29, 1995·Annals of the New York Academy of Sciences·S J LolaitM J Brownstein

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Citations

Jan 4, 2001·Molecular Genetics and Metabolism·H Shojo, Y Kaneko
Apr 14, 2000·American Journal of Obstetrics and Gynecology·M G ZlatnikM S Soloff
May 18, 1999·Frontiers in Neuroendocrinology·J G Verbalis
Sep 5, 2006·Brain Research·J Thomas CurtisZuoxin Wang
Apr 23, 2002·Biology of Reproduction·John A CoplandMelvyn S Soloff
Nov 1, 2018·Genes, Brain, and Behavior·Nicholas I CilzW Scott Young
Sep 11, 2010·Stress : the International Journal on the Biology of Stress·Ja RoperSj Lolait
Jul 29, 1998·Endocrinology·Y J JengM S Soloff
Jul 30, 2020·American Journal of Physiology. Cell Physiology·Dong-Dong GaoWen-Liang Zhou
May 12, 1998·The American Journal of Physiology·Z StrakovaM S Soloff
Mar 29, 2001·Physiological Reviews·G Gimpl, F Fahrenholz
Jul 15, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Yuan-Lin DongChandrasekhar Yallampalli
Jan 1, 2003·World Journal of Gastroenterology : WJG·Dong-Ping XieKe-Jing Liu

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