PMID: 8967342Apr 1, 1996Paper

Molecular cloning and functional expression of a sodium-dicarboxylate cotransporter from human kidney

The American Journal of Physiology
Ana M Pajor

Abstract

The renal Na(+)-dicarboxylate cotransporter reabsorbs Krebs cycle intermediates, such as succinate and citrate, from the glomerular filtrate. The present study describes the cloning and characterization of the human renal Na(+)-dicarboxylate cotransporter, hNaDC-1. The amino acid sequence of hNaDC-1 is 78% identical to the rabbit renal Na(+)-dicarboxylate cotransporter, NaDC-1, and 42% identical to the rat renal Na(+)-sulfate transporter, NaSi-1. The carboxy terminus of hNaDC-1 protein contains two N-glycosylation sites that appear to be utilized. Xenopus oocytes injected with hNaDC-1 cRNA expressed a low-affinity Na(+)-dependent dicarboxylate transporter with Michaelis constant (Km) for succinate around 0.4 mM. The transport of succinate by hNaDC-1 was insensitive to the pH of the medium, whereas the transporter of citrate was stimulated by acidic pH. Northern blot analysis indicates that hNaDC-1 mRNA is found in both kidney and intestine. The gene for hNaDC-1 was localized to chromosome 17. This study provides the first demonstration that the human kidney contains a low-affinity Na(+)-dicarboxylate cotransporter with properties that resemble those of the Na(+)-dicarboxylate cotransporter on the apical membrane of the rabbit r...Continue Reading

Citations

Oct 8, 2005·Pflügers Archiv : European journal of physiology·Ana M Pajor
Jul 15, 2000·American Journal of Physiology. Renal Physiology·X Yao, A M Pajor
May 12, 2000·American Journal of Physiology. Renal Physiology·A K MeinildE M Wright
Mar 11, 2000·American Journal of Physiology. Renal Physiology·K S Hering-SmithL L Hamm
May 4, 2000·American Journal of Physiology. Cell Physiology·H WangV Ganapathy
Jul 23, 2004·Physiological Reviews·Stephen H Wright, William H Dantzler
Jul 1, 2008·Applied and Environmental Microbiology·Haruhiko TeramotoHideaki Yukawa
Dec 26, 2018·Nutrients·Jessica ConnorsJohan Van Limbergen
Feb 20, 2004·American Journal of Physiology. Cell Physiology·Seiji ArugaRobert J Alpern
Jan 28, 2006·Planta·Takehiko ShimadaEduardo Blumwald

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