PMID: 11322891Apr 27, 2001Paper

Molecular cloning and functional expression of rat liver cytosolic acetyl-CoA hydrolase

European Journal of Biochemistry
N SuematsuF Isohashi

Abstract

A cytosolic acetyl-CoA hydrolase (CACH) was purified from rat liver to homogeneity by a new method using Triton X-100 as a stabilizer. We digested the purified enzyme with an endopeptidase and determined the N-terminal amino-acid sequences of the two proteolytic fragments. From the sequence data, we designed probes for RT-PCR, and amplified CACH cDNA from rat liver mRNA. The CACH cDNA contains a 1668-bp ORF encoding a protein of 556 amino-acid residues (62 017 Da). Recombinant expression of the cDNA in insect cells resulted in overproduction of functional acetyl-CoA hydrolase with comparable acyl-CoA chain-length specificity and Michaelis constant for acetyl-CoA to those of the native CACH. Database searching shows no homology to other known proteins, but reveals high similarities to two mouse expressed sequence tags (91% and 93% homology) and human mRNA for KIAA0707 hypothetical protein (50% homology) of unknown function.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M Kozak
Jul 6, 1989·Biochimica Et Biophysica Acta·Y NakanishiY Sakamoto
Feb 15, 1985·European Journal of Biochemistry·H D Söling, C Rescher
Oct 15, 1985·European Journal of Biochemistry·Y NakanishiY Sakamoto
Jun 12, 1986·Nature·L M SmithL E Hood
Nov 14, 1973·Nature: New Biology·I TinocoJ Bralla
Feb 5, 1973·Biochemical and Biophysical Research Communications·M R LakshmananJ W Porter
May 3, 1996·The Journal of Biological Chemistry·C G BroustasA K Hajra
Mar 6, 1997·Biochemical and Biophysical Research Communications·J YamadaT Suga
Sep 12, 1998·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·K IshikawaO Ohara
Dec 22, 1999·European Journal of Biochemistry·R Perham

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Citations

May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Naoya SuematsuFumihide Isohashi
Jul 9, 2011·PloS One·Ann-Gerd ThorsellLari Lehtiö
Jul 19, 2013·Cellular and Molecular Life Sciences : CMLS·Veronika TillanderStefan E H Alexson
May 18, 2010·Progress in Lipid Research·Brenda KirkbyJade K Forwood
Oct 1, 2013·Biochimie·Fabien Alpy, Catherine Tomasetto
Apr 2, 2008·Placenta·S AngelettiS Genti-Raimondi
Aug 17, 2005·Journal of Lipid Research·Mary C HuntStefan E H Alexson
May 28, 2013·Journal of Lipid Research·Yasuhiro HoribataHiroyuki Sugimoto
Oct 4, 2011·The Journal of Endocrinology·Barbara J Clark
Feb 28, 2003·The Journal of Biological Chemistry·Leh-Miauh BuuFang-Jen S Lee
May 2, 2003·The Journal of Biological Chemistry·Raymond E Soccio, Jan L Breslow
Aug 21, 2007·The Journal of Biological Chemistry·Keishi KannoDavid E Cohen
Jan 14, 2020·Molecular and Cellular Endocrinology·Barbara J Clark
May 18, 2020·Progress in Lipid Research·Crystall M D SwarbrickJade K Forwood
Jun 25, 2005·Journal of Cell Science·Fabien Alpy, Catherine Tomasetto

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